PURPOSE:The purpose of this work was to determine microtensile dentin bond strengths (microTBS) of dentin-resin composite bonds after three-month storage in artificial saliva containing either collagenase (COL) or cholesterol esterase (EST). The null hypothesis tested is that the resin-dentin bond strength is equivalent for each storage medium at the tested storage times. MATERIALS AND METHODS:Resin composite was bonded to occlusal dentin, and microTBS specimens were formed and stored in the artificial saliva, COL, EST, or synthetic oil. After 24 h and 12-week storage, microTBS was determined and failure modes were characterized by SEM. The interfacial ultrastructure was evaluated by transmission electron microscopy as unstained and stained sections (phosphotungstic acid/uranyl acetate). Statistical analysis was performed by ANOVA and Weibull survival analyses at the 0.05 level of statistical significance. RESULTS:There were significantly weaker bond strengths after 12 weeks for all experimental storage media (p < 0.001). Artificial saliva containing EST lowered bond strengths to a significantly greater extent than did COL after 12 weeks of storage, while no difference between these groups could be discerned after 24 h. Therefore, the null hypothesis of this experiment is rejected. CONCLUSION:Exogenous enzymatic challenge to resin-dentin bonds decreased bond durability only with EST. However, when further challenges to ideal infiltration of the comonomers into the hybrid layer were carried out using inadequate removal of solvent, additional alterations in hybrid layer ultrastructure were discerned by TEM that may represent different potential degradative processes. The contribution of endogenous enzymatic challenges to the primary degradative process, ie, hydrolysis, is unknown and deserves continued attention.

译文

目的:这项工作的目的是确定在含有胶原酶(COL)或胆固醇酯酶(EST)的人工唾液中储存三个月后牙本质-树脂复合键的微拉伸牙本质粘合强度(microTBS)。测试的原假设是在测试的存储时间下每种存储介质的树脂-牙本质粘合强度均相等。
材料与方法:将树脂复合材料粘结到牙本质牙本质上,形成microTBS标本并保存在人工唾液,COL,EST或合成油中。储存24小时和12周后,确定了microTBS,并通过SEM表征了失效模式。通过透射电子显微镜评价界面超微结构为未染色和染色的切片(磷钨酸/乙酸铀酰)。通过ANOVA和Weibull生存分析以0.05的统计显着性水平进行统计分析。
结果:所有实验存储介质在12周后粘合强度均显着降低(p <0.001)。贮藏12周后,含有EST的人工唾液与COL相比,其结合强度降低的幅度要大得多,而24小时后未发现这两组之间的差异。因此,该实验的原假设被拒绝了。
结论:外源酶对树脂-牙本质键的挑战仅通过EST降低了键的持久性。然而,当通过不充分去除溶剂对共聚单体向理想的渗透入杂化层的进一步挑战时,TEM可以看出杂化层超微结构的其他变化,这可能代表了不同的潜在降解过程。内源性酶挑战对主要降解过程(即水解)的贡献尚不清楚,值得继续关注。

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