Gastrointestinal nematode infections of livestock animals are prevalent and costly problems worldwide. Currently, infections are controlled by anthelmintic chemicals but increasing drug resistance has prompted research interest to shift towards alternative methods of control such as vaccine development and selection of worm-resistant animals. The present study analyses proteins from Trichostrongylus colubriformis infective L3s that are recognised by IgG of immune sheep. Following protein separation via two-dimensional electrophoresis and Western blot probing with plasma from sheep resistant to T. colubriformis, mass spectrometry-based proteomic analyses were used to identify immuno-reactive protein spots. We were able to identify 28 immune targets, including aspartyl protease inhibitor, enolase, chaperone proteins, galectin, glycolytic enzymes, kinase, phosphatase and structural muscle proteins such as myosin, paramyosin, calponin and DIM-1. The data suggest that immune responses to T. colubriformis are dispersed over a relatively large number of parasite antigens, including several cytoplasmically expressed proteins. The results have new implications for understanding the molecular mechanisms that underpin host-parasite interaction during gastrointestinal nematode infections.

译文

:家畜的胃肠道线虫感染是世界范围内普遍存在且代价高昂的问题。目前,感染是由驱虫药控制的,但耐药性的提高促使研究兴趣转向了替代控制方法,如疫苗开发和抗蠕虫动物的选择。本研究分析了来自毛支毛滴虫的感染性L3s的蛋白,这些蛋白被免疫羊的IgG识别。通过二维电泳分离蛋白质,并用血浆对来自粘膜衣原体的绵羊进行血浆蛋白质印迹法探测后,基于质谱的蛋白质组学分析被用于鉴定免疫反应性蛋白斑点。我们能够鉴定出28个免疫靶标,包括天冬氨酰蛋白酶抑制剂,烯醇酶,伴侣蛋白,半乳凝素,糖酵解酶,激酶,磷酸酶和结构性肌肉蛋白,如肌球蛋白,副肌球蛋白,钙蛋白和DIM-1。数据表明,对粘膜衣原体的免疫反应分散在相对大量的寄生虫抗原上,包括几种细胞质表达的蛋白质。该结果对理解胃肠道线虫感染过程中宿主与寄生虫相互作用的分子机制具有新的启示。

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