BACKGROUND & AIMS:
:In the present study, we examined the in vivo effects of estradiol (E(2)) and progesterone on cyclooxygenase (COX) 2, prostaglandin F synthase (PTGFS, also known as PGFS), and membrane-associated prostaglandin E synthase 1 (mPTGES1) expression at both mRNA and protein levels using a nonpregnant ovariectomized (OVX) sheep model. Sixteen ewes were OVX shortly after ovulation. After 40 days, ewes were treated with saline (Cont, n=5), or E(2) infused intravenously for 2 days (50 microg/day, n=5) or intravaginal progesterone (P) sponges for 10 days (0.3 g P, n=6). Cervical COX2, PTGFS, and mPTGES1 mRNA and protein were quantified by northern and western blot analyses respectively. In situ hybridization and/or immunocytochemistry were used to localize the cellular distribution of COX2, PTGFS, and mPTGES1 mRNAs and proteins. COX2 mRNA abundance increased significantly in the cervix after E(2) treatment (P<0.05). However, progesterone was a more potent stimulator than E(2) of COX2 mRNA and protein abundance in the cervix (P<0.01). In contrast, PTGFS and mPTGES1 mRNA and protein concentrations did not change after E(2) or progesterone treatment (P>0.05). COX2, PTGFS, and mPTGES1 mRNA and protein were only localized in cervical glandular epithelial cells. This study shows that increased cervical COX2 mRNA and protein, but not PTGFS and mPTGES1 mRNA and protein, were associated with E(2) and progesterone treatment in nonpregnant sheep. More strikingly, progesterone was a more potent stimulator of cervical COX2 expression than E(2). The expression of COX2, PTGFS, and mPTGES1 mRNA and/or protein was confined in the cervical glandular epithelial cells of nonpregnant sheep.
背景与目标:
: 在本研究中,我们检查了雌二醇 (E(2)) 和孕酮对环氧合酶 (COX) 2,前列腺素F合酶 (PTGFS,也称为PGFS) 的体内作用,使用非妊娠卵巢切除 (OVX) 绵羊模型在mRNA和蛋白质水平上的膜相关前列腺素E合酶1 (mPTGES1) 表达。排卵后不久,有16只母羊出现OVX。40天后,用盐水 (Cont,n = 5) 或E(2) 静脉内输注2天 (50微克/天,n = 5) 或阴道内孕酮 (P) 海绵治疗10天 (0.3g P,n = 6)。分别通过northern和western印迹分析定量子宫颈COX2,PTGFS和mPTGES1 mRNA和蛋白。原位杂交和/或免疫细胞化学用于定位COX2,ptgf和mPTGES1 mrna和蛋白质的细胞分布。E(2) 处理后子宫颈COX2 mRNA丰度显著增加 (P<0.05)。然而,孕酮是子宫颈中COX2 mRNA和蛋白质丰度的比E(2) 更有效的刺激因子 (P<0.01)。相反,在E(2) 或孕酮处理后,PTGFS和mPTGES1 mRNA和蛋白浓度没有变化 (P>0.05)。COX2,PTGFS和mPTGES1 mRNA和蛋白仅位于宫颈腺上皮细胞中。这项研究表明,在未怀孕的绵羊中,子宫颈COX2 mRNA和蛋白的增加,而PTGFS和mPTGES1 mRNA和蛋白的增加与E(2) 和孕酮治疗有关。更引人注目的是,孕酮比E(2) 更有效地刺激子宫颈COX2表达。COX2,PTGFS和mPTGES1 mRNA和/或蛋白的表达仅限于未怀孕绵羊的宫颈腺上皮细胞。