Compactness has been used to locate discontinuous structural units containing one or more polypeptide chains in proteins of known structure. Rather than exhaustively calculating the compactness of all possible units, our procedure uses a screening algorithm to find discontinuous regions that are potentially compact. Precise calculations of compactness are restricted only to units in these regions. With our procedure, compactness can be used to discover discontinuous domains with virtually any number of disjoint peptides. Small, single-domain proteins may contain several compact regionsthus, compact regions do not always correspond to folding domains.

Because a domain is an independent folding unit and should contain a hydrophobic core, compact units were further examined for the presence of hydrophobic clusters (Zehfus MH, 1995, Protein Sci 41188-1202). This added constraint limits the number of acceptable units and helps greatly in the location of the true structural domains. The larger hydrophobically stabilized compact units correspond to domains, while the smaller units may correspond to folding intermediates.

译文

紧密度已被用于在已知结构的蛋白质中定位包含一个或多个多肽链的不连续结构单元。我们的过程不是完全计算所有可能单元的紧凑性,而是使用筛选算法来查找可能紧凑的不连续区域。紧凑度的精确计算仅限于这些区域中的单位。通过我们的程序,紧密度可用于发现几乎任何数量的不连续肽的不连续域。小的单结构域蛋白可能包含几个紧密区域,因此,紧密区域并不总是对应于折叠结构域。

由于结构域是一个独立的折叠单元,应该包含疏水核心,因此需要进一步研究紧密结构单元的结构疏水簇的存在(Zehfus MH,1995,Protein Sci 41188-1202)。这种增加的限制限制了可接受单元的数量,并极大地帮助确定了真正的结构域。较大的疏水稳定致密单元对应于结构域,而较小的单元可能对应于折叠中间体。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录