In our screening program for antihypertensive plant constituents extracts of the leaves and bark of Badula barthesia showed strong in vitro inhibition of angiotensin converting enzyme (ACE). Rapanone (1), 2,5-dihydroxy-3-tridecyl-1,4-benzoquinone, was isolated as an active constituent of the leaves. The IC(50) values of rapanone and three 3-alkyl-2,5-dihydroxybenzoquinones, (2)-(4), (Z)-2,5-dihydroxy-3-(pentadec-8-enyl)-1,4-benzoquinone, (Z,Z)-2,5-dihydroxy-3-(heptadeca-8,11-dienyl)-1,4-benzoqui-none and (Z)-2,5-dihydroxy-3-(heptadec-8-enyl)-1,4-benzoquinone recently isolated from Embelia angustifolia were determined. The following IC(50) ± S.D. values have been obtained (1) 36 ± 4.6 μM, (2) 19 ± 6.2 μM, (3) 19 ± 8.7 μM and (4) 16 ± 3.0 μ.M. The IC(50) value for the reference compound Captopril was determined to 12 ± 2.6 nM. The antimicrobial activity of the four compounds was determined by thin layer chromatography agar overlay technique as minimum growth inhibitory amount in μg. One yeast, Candida albicans, and four bacteria, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus were used as test organisms.

译文

:在我们针对降压植物成分的筛选程序中,Badula barthesia的叶子和树皮提取物显示出对血管紧张素转化酶(ACE)的强烈抑制作用。分离出2,5-二羟基-3-十三烷基-1,4-苯醌的雷帕酮(1)作为叶片的活性成分。雷帕酮和三个3-烷基-2,5-二羟基苯醌,(2)-(4),(Z)-2,5-二羟基-3-(pentadec-8-enyl)-1, 4-苯醌,(Z,Z)-2,5-二羟基-3-(十七烷-8,11-二烯基)-1,4-苯并喹酮和(Z)-2,5-二羟基-3-(庚二酮)确定了最近从安哥拉花(Embelia angustifolia)分离的-8-烯基)-1,4-苯醌。以下IC(50)±S.D.已获得(1)36±4.6μM,(2)19±6.2μM,(3)19±8.7μM和(4)16±3.0μM。参考化合物卡托普利的IC(50)值确定为12±2.6 nM。通过薄层层析琼脂覆盖技术确定这四种化合物的抗微生物活性,以μg为单位的最小生长抑制量。将一种酵母,白色念珠菌和四种细菌(枯草芽孢杆菌,大肠杆菌,铜绿假单胞菌和金黄色葡萄球菌)用作测试生物。

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