High-throughput diversity amplicon sequencing of marine microbial samples has revealed that members of the Mamiellophyceae lineage are successful phytoplankton in many oceanic habitats. Indeed, these eukaryotic green algae can dominate the picoplanktonic biomass, however, given the broad expanses of the oceans, their geographical distributions and the phylogenetic diversity of some groups remain poorly characterized. As these algae play a foundational role in marine food webs, it is crucial to assess their global distribution in order to better predict potential changes in abundance and community structure. To this end, we analyzed the V9-18S small subunit rDNA sequences deposited from the Tara Oceans expedition to evaluate the diversity and biogeography of these phytoplankton. Our results show that the phylogenetic composition of Mamiellophyceae communities is in part determined by geographical provenance, and do not appear to be influenced - in the samples recovered - by water depth, at least at the resolution possible with the V9-18S. Phylogenetic classification of Mamiellophyceae sequences revealed that the Dolichomastigales order encompasses more sequence diversity than other orders in this lineage. These results indicate that a large fraction of the Mamiellophyceae diversity has been hitherto overlooked, likely because of a combination of size fraction, sequencing and geographical limitations.

译文

海洋微生物样品的高通量多样性扩增子测序表明,Mamiellophyceae谱系成员在许多海洋生境中都是成功的浮游植物。的确,这些真核绿藻可以主导微微浮游生物量,但是,鉴于海洋广阔,它们的地理分布和某些物种的系统发育多样性仍然很差。由于这些藻类在海洋食物网中起着基础性作用,因此至关重要的是评估它们的全球分布,以便更好地预测丰度和群落结构的潜在变化。为此,我们分析了塔拉海洋探险队沉积的V9-18S小亚基rDNA序列,以评估这些浮游植物的多样性和生物地理分布。我们的结果表明,Mamiellophyceae群落的系统发育组成部分地由地理来源决定,至少在V9-18S可能达到的分辨率下,似乎不受水深的影响-在回收的样品中。系统进化分类的Mamiellophyceae序列显示,与该谱系中的其他顺序相比,Dolichomastigales顺序包含更多的序列多样性。这些结果表明,迄今为止,由于大小比例,测序和地理限制的综合作用,沙门氏菌的大部分多样性都被忽略了。

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