Among the various biomaterials available for tissue engineering and therapeutic applications, microbial polyhydroxyalkanoates offer the most diverse range of thermal and mechanical properties. In this study, the biocompatibility of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB); containing 50 mol % of 4-hydroxybutyrate] copolymer produced by Delftia acidovorans was evaluated. The cytotoxicity, mode of cell death, and genotoxicity of P(3HB-co-4HB) extract against V79 and L929 fibroblast cells were assessed using MTT assay, acridine orange/propidium iodide staining, and alkaline comet assay, respectively. Our results demonstrate that P(3HB-co-4HB) treated on both cell lines were comparable with clinically-used Polyglactin 910, where more than 60% of viable cells were observed following 72-h treatment at 200 mg/mL. Further morphological investigation on the mode of cell death showed an increase in apoptotic cells in a time-dependent manner in both cell lines. On the other hand, P(3HB-co-4HB) at 200 mg/mL showed no genotoxic effects as determined by alkaline comet assay following 72-h treatment. In conclusion, our study indicated that P(3HB-co-4HB) compounds showed good biocompatibility in fibroblast cells suggesting that it has potential to be used for future medical applications.

译文

:在可用于组织工程和治疗应用的各种生物材料中,微生物聚羟基链烷酸酯具有最广泛的热和机械性能范围。在这项研究中,聚(3-羟基丁酸酯-co-4-羟基丁酸酯)[P(3HB-co-4HB);评价了由Delftia acidovorans生产的含有50mol%的4-羟基丁酸酯]共聚物的共聚物。使用MTT法,assay啶橙/碘化丙啶染色和碱彗星法分别评估了P(3HB-co-4HB)提取物对V79和L929成纤维细胞的细胞毒性,细胞死亡方式和遗传毒性。我们的结果表明,在两种细胞系上处理的P(3HB-co-4HB)与临床使用的Polyglactin 910相当,在200 mg / mL的72小时处理后观察到超过60%的活细胞。对细胞死亡方式的进一步形态学研究显示,两种细胞系中凋亡细胞均以时间依赖性方式增加。另一方面,200 mg / mL的P(3HB-co-4HB)在72小时的处理后通过碱彗星测定没有显示出遗传毒性作用。总之,我们的研究表明P(3HB-co-4HB)化合物在成纤维细胞中显示出良好的生物相容性,这表明它有潜力用于未来的医学应用。

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