OBJECTIVES:Gelatin, a degraded collagen, has been widely used as a scaffolding material in tissue engineering applications. In this work, we aimed at the development of in situ, cross-linking, cytocompatible hydrogels by the use of transglutaminase as a cross-linker for potential application in the regeneration of tissues.
METHODS:Hydrogels were prepared from gelatin of different concentrations and bloom degree (175 (G175) or 300 (G300) bloom gelatin) and cross-linked with various amounts of microbial transglutaminase (mTG) at 37°C. Mechanical properties and cross-linking degree were studied by rheology and swelling experiments. Four hydrogels with different stiffness were selected for studies with embedded human adipose-derived stem cells (hASCs).
RESULTS:Hydrogels were obtained with storage modulus (G') values between 11 (±1) Pa and 1,800 (±200) Pa with gelation times between 80 (±6) and 450 (±36) seconds. G300 cross-linked gelatin hydrogels displayed higher gel stiffness, lower swelling ratio and gelled more rapidly compared to the hydrogels prepared from G175. Stiffer hydrogels (50 and 200 Pa) showed greater ability to support the proliferation of hASCs than softer ones (11 and 30 Pa). The highest cell proliferation was observed with a hydrogel of 200 Pa modulus.
CONCLUSIONS:Overall, transglutaminase cross-linked gelatin hydrogels might be suitable as injectable hydrogels for the engineering of musculoskeletal and other types of connective tissues.
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【Mechanical properties and biocompatibility of in situ enzymatically cross-linked gelatin hydrogels.].
【原位酶交联明胶水凝胶的机械性能和生物相容性。】
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DOI：10.5301/ijao.5000553文章类型：杂志文章
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目的：明胶是一种降解的胶原蛋白，已被广泛用作组织工程应用中的支架材料。在这项工作中，我们旨在通过使用转谷氨酰胺酶作为交联剂开发原位，交联，细胞相容性水凝胶，以潜在地应用于组织再生。
方法：由不同浓度和水华程度的明胶（175（G175）或300（G300）水华明胶）制备水凝胶，并在37°C下与各种量的微生物转谷氨酰胺酶（mTG）交联。通过流变学和溶胀实验研究了力学性能和交联度。选择了四种具有不同刚度的水凝胶用于嵌入式人脂肪衍生干细胞（hASC）的研究。
结果：获得的水凝胶的储能模量（G'）值为11（±1）Pa至1,800（±200）Pa，胶凝时间为80（±6）至450（±36）秒。与从G175制备的水凝胶相比，G300交联的明胶水凝胶显示出更高的凝胶刚度，更低的溶胀率和更快地凝胶化。较坚硬的水凝胶（50和200 Pa）显示出比较软的水凝胶（11和30 Pa）更大的支持hASCs增殖的能力。用200 Pa模量的水凝胶观察到最高的细胞增殖。
结论：总体而言，转谷氨酰胺酶交联的明胶水凝胶可能适合作为注射水凝胶用于肌肉骨骼和其他类型结缔组织的工程改造。
方法：由不同浓度和水华程度的明胶（175（G175）或300（G300）水华明胶）制备水凝胶，并在37°C下与各种量的微生物转谷氨酰胺酶（mTG）交联。通过流变学和溶胀实验研究了力学性能和交联度。选择了四种具有不同刚度的水凝胶用于嵌入式人脂肪衍生干细胞（hASC）的研究。
结果：获得的水凝胶的储能模量（G'）值为11（±1）Pa至1,800（±200）Pa，胶凝时间为80（±6）至450（±36）秒。与从G175制备的水凝胶相比，G300交联的明胶水凝胶显示出更高的凝胶刚度，更低的溶胀率和更快地凝胶化。较坚硬的水凝胶（50和200 Pa）显示出比较软的水凝胶（11和30 Pa）更大的支持hASCs增殖的能力。用200 Pa模量的水凝胶观察到最高的细胞增殖。
结论：总体而言，转谷氨酰胺酶交联的明胶水凝胶可能适合作为注射水凝胶用于肌肉骨骼和其他类型结缔组织的工程改造。
