Plant pathogenic Pseudomonas syringae produce the hydroxy-β-lactam antimetabolite tabtoxinine-β-lactam (TβL) as a time-dependent inactivating glutamine analogue of plant glutamine synthetases. The producing pseudomonads use multiple modes of self-protection, two of which are characterized in this study. The first is the dipeptide ligase TblF which converts tabtoxinine-β-lactam to the TβL-Thr dipeptide known as tabtoxin. The dipeptide is not recognized by glutamine synthetase. This represents a Trojan Horse strategy: the dipeptide is secreted, taken up by dipeptide permeases in neighboring cells, and TβL is released by peptidase action. The second self-protection mode is elaboration by the acetyltransferase Ttr, which acetylates the α-amino group of the proximal inactivator TβL, but not the tabtoxin dipeptide.

译文

植物致病性丁香假单胞菌可产生羟基-β-内酰胺抗代谢物毒素-β-内酰胺(TβL),作为植物谷氨酰胺合成酶的时间依赖性灭活谷氨酰胺类似物。产生的假单胞菌使用多种自我保护模式,本研究表征了其中两种。第一个是二肽连接酶TblF,它将Tabtoxinine-β-lactam转化为称为Tabtoxin的TβL-Thr二肽。谷氨酰胺合成酶不能识别二肽。这代表了特洛伊木马策略:二肽被分泌,被邻近细胞中的二肽渗透酶吸收,而TβL通过肽酶的作用释放。第二种自我保护模式是通过乙酰基转移酶Ttr进行的,该酶可乙酰化近端灭活剂TβL的α-氨基,但不能使毒素二肽乙酰化。

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