A novel lectin was purified from the fruiting bodies of king bolete mushrooms (Boletus edulis, also called porcino, cep or penny bun). The lectin was structurally characterized i.e its amino acid sequence and three-dimensional structure were determined. The new protein is a homodimer and each protomer folds as β-trefoil domain and therefore we propose the name Boletus edulis lectin (BEL) β-trefoil to distinguish it from the other lectin that has been described in these mushrooms. The lectin has potent anti-proliferative effects on human cancer cells, which confers to it an interesting therapeutic potential as an antineoplastic agent. Several crystal forms of the apoprotein and of complexes with different carbohydrates were studied by X-ray diffraction. The structure of the apoprotein was solved at 1.12 Å resolution. The interaction of the lectin with lactose, galactose, N-acetylgalactosamine and T-antigen disaccharide, Galβ1-3GalNAc, was examined in detail. All the three potential binding sites present in the β-trefoil fold are occupied in at least one crystal form and are described in detail in this paper. No important conformational changes are observed in the lectin when comparing its co-crystals with carbohydrates with those of the ligand-free protein.

译文

:从牛肝菌王菇(Boletus edulis,也称为牛肝菌,cep或便士面包)的子实体中纯化出一种新型凝集素。对凝集素进行结构表征,即确定其氨基酸序列和三维结构。新蛋白是同源二聚体,每个前体折叠成β-三叶结构域,因此我们提出了牛肝菌凝集素(BEL)β-三叶的名称,以将其与这些蘑菇中描述的其他凝集素区分开。所述凝集素对人癌细胞具有有效的抗增殖作用,使其具有作为抗肿瘤剂的令人感兴趣的治疗潜力。通过X射线衍射研究了载脂蛋白的几种晶体形式以及与不同碳水化合物的复合物。载脂蛋白的结构以1.12Å的分辨率解析。详细检查了凝集素与乳糖,半乳糖,N-乙酰半乳糖胺和T抗原二糖Galβ1-3GalNAc的相互作用。 β-三叶折叠中存在的所有三个潜在结合位点均以至少一种晶体形式占据,并在本文中进行了详细描述。当将其与碳水化合物的共晶体与不含配体的蛋白质的共晶体进行比较时,在凝集素中未观察到重要的构象变化。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录