The genomes of African swine fever virus isolates collected from soft ticks (Ornithodoros moubata) inhabiting warthog burrows in four areas of Zambia were compared by restriction enzyme site mapping. Isolates from different areas showed considerable diversity. The regions of genomes that differed between isolates were distributed throughout the virus genome, although some more conserved regions were identified, such as the right-hand third of the genome. The genomes of seven isolates from neighbouring warthog burrows within Livingstone Game Park in southern Zambia were more similar to each other than those from different areas. However, a number of differences were observed even between the genomes of isolates from the same warthog burrow. The variation between these latter isolates probably resulted from point mutations located at various positions along the genome, in addition to small additions or deletions at both terminal regions. Restriction enzyme site mapping indicated that one isolate may have originated by earlier recombination between two distinguishable viruses.

译文

:通过限制性内切酶位点图谱比较了从赞比亚四个地区疣猪洞穴中的软壁虱(Ornithodoros moubata)收集的非洲猪瘟病毒分离株的基因组。来自不同地区的分离株显示出相当大的多样性。尽管鉴定出一些更保守的区域,例如基因组的右手三分之一,但分离株之间的基因组区域却分布在整个病毒基因组中。赞比亚南部利文斯通野生动物园内邻近疣猪洞穴的七个分离株的基因组彼此之间的相似性高于不同地区。然而,甚至在来自相同疣猪洞穴的分离株的基因组之间也观察到许多差异。后两个分离物之间的变异可能是由于位于两个基因组各个位置的点突变,以及两个末端区域的少量添加或缺失所致。限制性酶切位点作图表明,一种分离物可能是由两种可区分的病毒之间的早期重组引起的。

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