The negative effects of ambient ultraviolet (UVA) on the water environment have been recently highlighted; UVA can create deleterious effects by stimulating stress on pelagic organisms. Little is known about UVA effects on oocyte characteristics of female fish. In the present study we explored the effects of exposure to ecologically relevant levels of simulated UVA radiation on ovaries of two major strains WT (HdrR) and P53 (-/-) of medaka (Oryzias latipes) mature female. Fish were assigned to control and three UVA-exposed groups as (15 min, 30 min, and 60 min/day) for three days and sample selection was 24 h and 14 days after exposure. Histological alterations and oocyte atresia percentage were analyzed in the UVA-exposed fish compared to control. Alteration comprised hyperthrophied follicular cells with increased thickness, breakdown of egg chorion (zona radiata), damage of cortical alveoli, and distorted nucleus and cytoplasm. The atresia percentages significantly increased with higher UVA exposure dose and time for both the wild type and the p53 deficient fish. The wild type displayed significantly higher oocyte atresia percentage than the p53 mutant. These results suggested that UVA exposure provoked histological alterations in both p53 and WT medaka oocytes leading to follicular atresia, which reduce female reproductive ability and larval production. UVA oocyte response showed p53 dependent and independent histological alteration, however, the p53 mutant was less sensitive to UVA than the wild type in medaka fish.

译文

最近强调了环境紫外线 (UVA) 对水环境的负面影响; UVA可以通过刺激对中上层生物的压力来产生有害影响。关于UVA对雌鱼卵母细胞特征的影响知之甚少。在本研究中,我们探索了暴露于模拟UVA辐射的生态相关水平对medaka (Oryzias latipes) 成熟雌性的两种主要菌株WT (HdrR) 和P53 (-/-) 的卵巢的影响。鱼被分配到对照组和三个UVA暴露组 (15 min,30 min和60 min/天),持续三天,样品选择为暴露后24 h和14 d。与对照组相比,分析了暴露于UVA的鱼的组织学改变和卵母细胞闭锁百分比。改变包括厚度增加的高throphied滤泡细胞,绒毛膜破裂 (辐射带),皮质肺泡受损以及细胞核和细胞质扭曲。野生型和p53缺陷鱼的闭锁百分比随UVA暴露剂量和时间的增加而显着增加。野生型的卵母细胞闭锁率明显高于p53突变体。这些结果表明,UVA暴露会引起p53和WT medaka卵母细胞的组织学改变,从而导致卵泡闭锁,从而降低女性的生殖能力和幼虫的产生。UVA卵母细胞反应显示出p53依赖性和独立的组织学改变,但是,在the鱼中,p53突变体对UVA的敏感性低于野生型。

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