The extracellular polymeric substances (EPSs) secreted by Bacillus flexus (GU592213) were estimated to have the molecular weight of approximately 1528 and 33,686 kDa with the elemental composition of Na, P, Mg, C, O, Cl and S. The (1)H NMR and FT-IR analysis of EPS confirmed the presence of different aliphatic and aromatic groups. The EPS was amorphous in nature with an average particle size of 13.969 μm (d 0.5) and roughness of 193 nm. The GC-MS analysis has revealed different monosaccharides such as fucose, ribose, xylose, galactose, mannose and glucose. Oligo and polysaccharides were detected with MALDI TOF-TOF MS. The bacterial EPS for the first time tested as a natural substratum for settle of zoospores of Ulva fasciata by incubating for various durations ranging from 2h to 48 h. The zoospore settlement on EPS coated cover slips progressively increased with incubation time in axenic cultures over controls. The EPS, thus investigated in this study was found to facilitate the primary settlement of spores that play crucial role in recruitment of macroalgal communities in coastal environment including intertidal regions.

译文

由弯曲芽孢杆菌 (GU592213) 分泌的细胞外聚合物 (EPSs) 估计具有约1528和33,686 kDa的分子量,其中元素组成为Na,P,Mg,C,O,Cl和S。EPS的 (1)H NMR和ft-ir分析证实了不同的脂肪族和芳香族基团的存在。EPS本质上是无定形的,平均粒径为13.969微米 (d 0.5),粗糙度为193纳米。Gc-ms分析显示了不同的单糖,例如岩藻糖,核糖,木糖,半乳糖,甘露糖和葡萄糖。用MALDI TOF-TOF MS检测寡核苷酸和多糖。通过孵育2小时至48小时的各种持续时间,首次将细菌EPS作为天然基质进行测试,以沉降Ulva fasciata游动孢子。与对照组相比,在无菌培养物中,EPS涂层盖玻片上的游动孢子沉降随着孵育时间的增加而逐渐增加。在本研究中如此调查的EPS被发现有助于孢子的主要沉降,这些孢子在包括潮间带地区在内的沿海环境中大型藻类群落的募集中起着至关重要的作用。

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