Candida glabrata is one of the most frequent organisms isolated from superficial and invasive fungal infections, after Candida albicans. This organism also exhibits intrinsically low susceptibility to azole antifungals and treatment often fails. The microdilution method is not very practical for use in routine susceptibility testing in the clinical laboratory, thus necessitating the use of other methods. In this study, we compared the in vitro activity of five antifungal agents in three different groups (echinocandin, polyene and azole) against 50 C. glabrata isolates by broth microdilution and disk diffusion methods recommended by Clinical Laboratory Standards Institute CLSI M27-A3 and CLSI M44-A, respectively. All the isolates were susceptible to amphotericin B (100%) and 98% of the isolates were susceptible to caspofungin by the broth microdilution method. Within the azole group drugs, voriconazole was the most active followed by fluconazole and itraconazole in vitro. The highest rate of resistance was obtained against itraconazole with a high number of isolates defined as susceptible-dose dependent or resistant. Although the disk diffusion method is easy to use in clinical laboratories, it shows very poor agreement with the reference method for fluconazole and itraconazole against C. glabrata (8% and 14%, respectively).

译文

光滑念珠菌是继白色念珠菌之后从浅表和侵袭性真菌感染中分离出来的最常见的生物之一。这种生物对唑类抗真菌药的敏感性本质上也较低,并且治疗通常失败。微量稀释法在临床实验室的常规药敏试验中使用不是很实用,因此需要使用其他方法。在这项研究中,我们通过临床实验室标准研究所CLSI M27-A3和CLSI M44-A推荐的肉汤微量稀释和纸片扩散方法,比较了三种不同组 (棘皮菌素,多烯和唑) 对50种无毛念珠菌的体外活性。通过肉汤微量稀释法,所有分离株均对两性霉素b (100%) 敏感,98% 分离株对卡泊芬净敏感。在唑类药物中,伏立康唑是最活跃的,其次是氟康唑和伊曲康唑。对伊曲康唑的耐药率最高,其中大量分离株被定义为敏感剂量依赖性或耐药性。尽管纸片扩散法易于在临床实验室中使用,但与氟康唑和伊曲康唑对光滑C的参考方法 (分别为8% 和14%) 的一致性很差。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录