A quantitative t.l.c. densitometric method for the simultaneous determination of the water-soluble vitamins B1, B2, B6, and B12 is proposed. The four vitamins are determined on one and the same plate by direct densitometry of the t.l.c. spots. A preliminary separation of the vitamins one from another, as well as a preliminary separation from other pharmaceuticals is not necessary. The determination is performed quickly and easily. The relative standard deviations of a single determination are the followingfor thiamine 6,6%, for riboflavin 5,5%, for pyridoxine 6,4%, and for cyanocobalamin 5,8%.

The proposed method is applied to the determination of B-vitamins in three pharmaceutical preparationsampoules B12 with B1, ampoules B-complex, and antisclerol dragee. The presence of other pharmaceuticals (vitamins C and PP, urethane, aminophenazone, phytin, rutin, theobromine, etc.) does not interfere with the determination.

译文

定量t.l.c.提出了同时测定水溶性维生素B1,B2,B6和B12的光密度法。通过t.l.c.斑点的直接密度测定法在同一平板上确定四种维生素。没有必要将维生素与其他药物进行初步分离,也没有必要与其他药物进行初步分离。该测定是快速而容易地进行的。硫胺素6,6%,核黄素5,5%,吡哆醇6,4% 和氰钴胺5,8% 的单次测定的相对标准偏差如下。
所提出的方法用于测定三种药物制备样品B12中的b族维生素,和反硬化剂糖衣。其他药物 (维生素c和PP,氨基甲酸酯,氨基苯甲酮,植素,芦丁,可可碱等) 的存在不会干扰测定。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录