We investigated the effects of vitamins C and E on the delayed-rectifier potassium current (IK(DR)), which is important in repolarizing the membrane potential, and on the transient A-type potassium current (IK(A)), which regulates neuronal firing frequency. The whole-cell patch-clamp technique was used to measure the currents from cultured Drosophila neurons derived from embryonic neuroblasts. The membrane potential was stepped to different voltages between -40 and +60 mV from a holding potential of -80 mV. IK(DR) and IK(A) measured in the vitamin C-containing solution (IK(DR) 305 +/- 16 pA, IK(A) 11 +/- 2 pA) were smaller than those measured in the control solution (488 +/- 21 pA, IK(A )28 +/- 3 pA). By contrast, IK(DR) and IK(A) measured in the vitamin E-containing solution (IK(DR) 561 +/- 21 pA, IK(A )31 +/- 3 pA) were greater than those measured in the control solution (422 +/- 15 pA, 17 +/- 2 pA). These results indicate that vitamins C and E can modulate potassium current amplitudes and possibly lead to altered neuronal excitability.

译文

我们研究了维生素c和E对延迟整流钾电流 (IK(DR)) 的影响,该电流对复极化膜电位很重要,并且对瞬时a型钾电流 (IK(A)) 的影响,调节神经元的放电频率。全细胞膜片钳技术用于测量来自胚胎成神经细胞的培养果蝇神经元的电流。将膜电位从-80 mV的保持电位步进到-40和 + 60 mV之间的不同电压。在含维生素c溶液 (IK(DR) 305 +/- 16 pA,IK(A) 11 +/- 2 pA) 中测得的IK(DR) 和IK(A) 小于在对照溶液中测得的IK (488 +/- 21 pA,IK(A )28 +/- 3 pA)。相比之下,在含维生素e溶液 (IK(DR) 561 +/- 21 pA,IK(A )31 +/- 3 pA) 中测得的IK(DR) 和IK(A) 大于在对照溶液中测得的IK (422 +/- 15 pA,17 +/- 2 pA)。这些结果表明,维生素c和E可以调节钾电流幅度,并可能导致神经元兴奋性改变。

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