Renal involvement in visceral leishmaniasis (VL) is very frequent but the pathogenesis of this nephropathy is poorly understood. In previous studies using dogs with VL we have detected new immunopathological elements in the glomeruli such as T cells and adhesion molecules. Although Leishmania (Leishmania) chagasi-infected dogs and hamsters are considered to be good models for VL, their use is limited for immunopathologic studies. The use of isogenic mouse strains susceptible to L. (L.) chagasi infection was an alternative but, on the other hand, the renal lesions of these animals have not yet been characterized. Thus, our purpose in the present study was to characterize mice infected with L. (L.) chagasi as a suitable model to study VL nephropathy. Kidney samples were obtained from control mice (N = 12) and from BALB/c mice (N = 24) injected intraperitoneally with 20 million L. (L.) chagasi amastigotes 7, 15, and 30 days after injection and processed for histopathological studies and detection of IgG deposits. Glomerular hypercellularity was clearly visible and, upon Mason's trichrome and periodic acid methenamine silver staining, a pattern suggestive of mesangial proliferative glomerulonephritis was observed in mice with VL. Time-dependent IgG deposits were also seen in infected mice. We consider L. (L.) chagasi-infected mice to be a suitable model for studies of the immunopathogenesis of glomerular lesions in VL.

译文

肾脏受累于内脏利什曼病 (VL) 非常常见,但对这种肾病的发病机理知之甚少。在先前使用VL狗的研究中,我们已经在肾小球中检测到新的免疫病理元素,例如T细胞和粘附分子。尽管利什曼原虫 (Leishmania) chagasi感染的狗和仓鼠被认为是VL的良好模型,但它们的使用仅限于免疫病理学研究。使用易感L的等基因小鼠品系。(L.) chagasi感染是另一种选择,但另一方面,这些动物的肾脏病变尚未得到表征。因此,我们在本研究中的目的是表征感染L的小鼠。(L.) chagasi作为研究VL肾病的合适模型。从对照小鼠 (N = 12) 和腹腔注射2000万L的BALB/c小鼠 (N = 24) 获得肾脏样品。(L。) 注射后7、15和30天的chagasi amastigotes,并进行组织病理学研究和IgG沉积物检测。肾小球细胞增生清晰可见,在Mason的三色和高碘酸甲胺银染后,在VL小鼠中观察到提示系膜增生性肾小球肾炎的模式。在感染的小鼠中也看到了时间依赖性的IgG沉积物。我们认为L。(L。) chagasi感染的小鼠是研究VL肾小球病变免疫发病机理的合适模型。

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