The immune-releasing effects of L-glutamine (Gln) supplementation in duck plague virus (DPV)-infected ducklings were evaluated in 120 seven-day-old ducklings that were divided into 8 groups. The ducklings in control and DPV, 0.5Gln and DPV + 0.5Gln, 1.0Gln and DPV + 1.0Gln, and 2.0Gln and DPV + 2.0Gln received 0, 0.5, 1.0, and 2.0 g of Gln/kg feed/d by gastric perfusion, respectively. Then, the ducklings in control to 2.0Gln were injected with 0.2 mL of phosphate-buffered saline, while those in DPV to DPV + 2.0Gln were injected with DPV at 0.2 mL of 2000 TCID50 (50% tissue culture infection dose) 30 minutes after gavage with Gln, sampled at 12 hours and days 1, 2, 4, and 6. Glutamine supplementation under physiological conditions enhanced immune function and toll-like receptor 4 (TLR4) expressions in a dose-dependent manner. An increase in Gln supplementation under DPV-infected conditions enhanced growth performance, decreased immunoglobulin (Ig) release in plasma and secretory IgA in the duodenum, ameliorated plasma cytokine levels, and suppressed overexpressions of the TLR4 pathway in the duodenum. The positive effects of Gln on the humoral immunity- and intestinal inflammation-related damage should be considered a mechanism by which immunonutrition can assist in the recovery from DPV infection.

译文

在120 7天大的小鸭中评估了L-谷氨酰胺 (Gln) 补充对鸭瘟病毒 (DPV) 感染的小鸭的免疫释放作用,这些小鸭分为8组。对照组和DPV,0.5Gln和DPV + 0.5Gln,1.0Gln和DPV + 1.0Gln和2.0Gln和DPV + 2.0Gln的雏鸭分别通过胃灌注获得0、0.5、1.0和2.0g Gln/kg饲料/d。然后,将对照至2.0Gln的小鸭注射0.2毫升磷酸盐缓冲盐水,而将DPV至DPV + 2.0Gln的小鸭在TCID50 (50% 组织培养感染剂量) 灌胃30分钟后,在12小时和第1、2天取样,以0.2毫升2000年DPV注射DPV,4,和6。生理条件下补充谷氨酰胺以剂量依赖的方式增强免疫功能和toll样受体4 (TLR4) 的表达。在DPV感染的条件下补充Gln的增加可增强生长性能,降低十二指肠血浆和分泌型IgA中的免疫球蛋白 (Ig) 释放,改善血浆细胞因子水平,并抑制十二指肠TLR4途径的过表达。Gln对体液免疫和肠道炎症相关损害的积极作用应被认为是免疫消耗有助于从DPV感染中恢复的机制。

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