Infectious pancreatic necrosis virus (IPNV), a type species of aquabirnaviruses in the family Birnaviridae, is an etiological agent of infectious pancreatic necrosis and has been isolated from epizootics of cultured salmonids. In the present study, an epithelioma papulosum cyprini (EPC) cell line persistently infected with IPNV (PI-EPC) was experimentally established by subculturing EPC cells surviving IPNV infection, and was characterized. PI-EPC cells were morphologically indistinguishable from EPC, but continued to grow and yield IPNV. PI-EPC cells showed no cytopathic effect due to IPNV inoculation, and susceptibility of PI-EPC cells against heterologous viruses was not different from that of EPC cells. Only one cell of 10(3.5) PI-EPC cells produced IPNV at approximately 10(0.5) 50% tissue culture infectious dose (TCID50)/cell/day, which was approximately 1,000 times lower than that of normal EPC cells. PI-EPC cells that did not yield IPNV (N-PI-EPC) were screened. The IPNV genome was detected from both PI-EPC and N-PI-EPC cells, and the IPNV VP2 structural protein was detected from both cell lines, but no other IPNV proteins were observed by Western blot analysis with anti-IPNV serum. Thus, multiplication of IPNV in PI-EPC cells was regulated by some host cell factors, except interferon.

译文

传染性胰腺坏死病毒 (IPNV) 是Birnaviridae家族中的一种aquabirna病毒,是传染性胰腺坏死的病原体,已从培养的鲑鱼的流行病中分离出来。在本研究中,通过传代培养存活IPNV感染的EPC细胞,实验建立了持续感染IPNV (pi-epc) 的丘疹上皮瘤 (EPC) 细胞系,并进行了表征。Pi-epc细胞在形态上与EPC没有区别,但继续生长并产生IPNV。由于IPNV接种,pi-epc细胞没有细胞病变作用,并且pi-epc细胞对异源病毒的敏感性与EPC细胞没有差异。10个 (3.5) pi-epc细胞中只有一个细胞产生约10个 (0.5) 50% 组织培养感染剂量 (TCID50)/细胞/天的IPNV,这比正常EPC细胞低约1,000倍。筛选了未产生IPNV (n-pi-epc) 的PI-EPC细胞。从PI-EPC和N-PI-EPC细胞中检测到IPNV基因组,从两种细胞系中检测到IPNV VP2结构蛋白,但通过抗IPNV血清的Western blot分析未观察到其他IPNV蛋白。因此,除干扰素外,pi-epc细胞中IPNV的增殖受某些宿主细胞因子的调节。

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