Thirteen cultivars of hop (Humulus lupulus L.) were tested by reverse transcription-polymerase chain reaction (RT-PCR) for the presence of apple mosaic virus (ApMV). The virus was detected in various amounts in all tested cultivars. Control hop clones derived from tissue cultures, treated by thermotherapy and maintained in greenhouse were virus-free. The procedure for sample preparation and RT-PCR of ApMV is described in detail.

译文

通过逆转录聚合酶链反应 (rt-pcr) 测试了13个啤酒花品种 (Humulus lupulus L.) 是否存在苹果花叶病毒 (ApMV)。在所有测试品种中均检测到不同数量的病毒。来自组织培养物的对照啤酒花克隆,通过热疗处理并保存在温室中,无病毒。详细介绍了ApMV的样品制备和rt-pcr程序。

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