The effects of laser light on the cellular DNA have not been extensively characterized. Low-power laser sources, such as the helium-neon (He-Ne) laser with a wavelength of 632.8 nm, have been found to produce photobiological and photodamage effects with evidence of interference with cell replication. We have investigated the effects of He-Ne laser irradiation on sister chromatid exchange (SCE) frequencies in sheep peripheral blood mononuclear cells (PBMC). Cultured cells were irradiated once at 6 selected energy intensities of laser irradiation and then stimulated with pokeweed mitogen and cultured in the presence of 5-bromodeoxyuridine (BrdUrd). The frequency of SCEs of both irradiated and non-irradiated cells were analyzed. The mean SCE of irradiated cells significantly increased with growing energy density up to a laser dose of 24 J/cm2, whereas after an energy density of 24 J/cm2, the SCE frequency decreased with increasing energy densities. We concluded that the application of He-Ne laser irradiation at energy densities ranging from 2 to 96 J/cm2 produced a different effect on SCE frequency in sheep PBMC in vitro.

译文

激光对细胞DNA的影响尚未得到广泛表征。已发现低功率激光源,例如波长为632.8 nm的氦氖 (He-Ne) 激光器,可产生光生物学和光损伤效应,并具有干扰细胞复制的证据。我们已经研究了He-Ne激光照射对绵羊外周血单核细胞 (PBMC) 中姐妹染色单体交换 (SCE) 频率的影响。以6种选定的激光照射能量强度照射培养的细胞一次,然后用商陆有丝分裂原刺激并在5-溴脱氧尿苷 (BrdUrd) 存在下培养。分析了辐照和非辐照细胞的sce频率。辐照细胞的平均SCE随着能量密度的增长而显着增加,直到激光剂量为24 J/cm2,而在能量密度为24 J/cm2之后,SCE频率随着能量密度的增加而降低。我们得出的结论是,在2至96 J/cm2的能量密度下应用He-Ne激光辐照对体外绵羊PBMC的SCE频率产生了不同的影响。

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