To examine the correlations of miR-24 expression in peripheral plasma with the onset of diabetic foot ulcer (DFU) and diabetic foot osteomyelitis (DFO) in type 2 diabetes mellitus (T2DM) patients and explore the clinical value of miR-24 as a potential biomarker for the diagnosis and treatment outcomes of DFU and DFO, a total of 60 newly diagnosed T2DM patients without DFU (T2DM group), 112 T2DM patients with DFU (DFU group), and 60 healthy controls (NC group) were included. DFU group were further divided into DFO group (n = 64) and non-DFO group (n = 48). MiR-24 levels were determined by quantitative real-time PCR, while clinical features and risk factors of DFU and DFO were explored. The expression level of miR-24 in T2DM and DFU group was significantly lower than in NC group (P < .05), and that in DFU group was significantly lower than in T2DM group (P < .01). Additionally, the level of miR-24 significantly decreased in DFO group compared to non-DFO group (P < .01). Moreover, it was negatively correlated with the amputation rate in DFU group (P = .043) and positively correlated with healing rate after 8 weeks (P = .036). The multivariate logistic regression analysis confirmed that a low expression of miR-24 was an independent risk factor for DFU and DFO. The ROC curve analysis indicated that the AUC of miR-24 for the diagnosis of DFU and DFO was 0.849 (95% CI, 0.618-0.879, P < .001) and 0.782 (95% CI, 0.595-0.813, P < .001). Thus, a decreased expression of miR-24 of T2DM patients was closely related to the occurrence, development and prognosis of DFU and DFO, suggesting the use of miR-24 as a potential biomarker for the prediction of DFU and DFO.

译文

目的: 研究2型糖尿病 (T2DM) 患者外周血浆miR-24表达与糖尿病足溃疡 (DFU) 和糖尿病足骨髓炎 (DFO) 发病的相关性,探讨miR-24作为潜在生物标志物对DFU和DFO的诊断和治疗结果的临床价值。纳入60例无DFU的新诊断T2DM患者 (T2DM组) 、112例伴有DFU的T2DM患者 (DFU组) 和60例健康对照者 (NC组)。DFU组分为DFO组 (n = 64) 和非DFO组 (n = 48)。采用实时荧光定量PCR检测MiR-24水平,探讨DFU和DFO的临床特征及危险因素。T2DM组和DFU组miR-24表达水平显著低于NC组 (p  < .05),DFU组表达水平显著低于T2DM组 (p  < .01)。此外,与非DFO组相比,DFO组的miR-24水平显著降低 (p  < .01)。DFU组与截肢率呈负相关 (P = .043),与术后8周愈合率呈正相关 (P = .036)。多因素logistic回归分析证实,miR-24低表达是DFU和DFO的独立危险因素。ROC曲线分析表明,miR-24诊断DFU和DFO的AUC为0.849 (95% CI,0.618-0.879,p  < .001) 和0.782 (95% CI,0.595-0.813,p  < .001)。因此,T2DM患者miR-24表达下降与DFU和DFO的发生、发展及预后密切相关,提示miR-24作为预测DFU和DFO的潜在生物标志物。

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