Type III protein secretion systems, which are organelles with the capacity to deliver bacterial proteins into host cells, have been adapted to deliver heterologous antigens for vaccine development. A limitation of these antigen delivery systems is that some proteins are not amenable to secretion through this pathway. We show here that proteins from the simian and human immunodeficiency viruses that are not permissive for secretion through a Salmonella enterica serovar Typhimurium type III secretion system can be modified to travel this secretion pathway by introduction of discrete mutations. Proteins optimized for secretion were presented more efficiently via the major histocompatibility complex class I pathway and were able to induce a better immune response.

译文

III型蛋白质分泌系统是具有将细菌蛋白质递送到宿主细胞中的能力的细胞器,已适应为疫苗开发提供异源抗原。这些抗原递送系统的局限性在于某些蛋白质不适合通过该途径分泌。我们在这里显示,来自猿猴和人类免疫缺陷病毒的蛋白质不允许通过沙门氏菌血清型鼠伤寒III型分泌系统分泌,可以通过引入离散突变来改变这种分泌途径。通过主要的组织相容性复合体I类途径更有效地呈现了针对分泌进行优化的蛋白质,并且能够诱导更好的免疫反应。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录