Mitochondrial dysfunction is part of many pathological states in patients, such as sepsis or stroke. Presently, the monitoring of mitochondrial function in patients is extremely rare, even though NADH redox state is routinely measured in experimental animals. In this article, we describe the scientific backgrounds and practical use of mitochondrial NADH fluorescence measurement that was applied to patients in the past few years. In addition to NADH, we optically measured the microcirculatory blood flow and volume, as well as HbO(2) oxygenation, from the same tissue area. The four detected parameters provide real time data on tissue viability, which is critical for patients monitoring.

译文

线粒体功能障碍是患者许多病理状态的一部分,如败血症或中风。目前,即使在实验动物中常规测量NADH氧化还原状态,对患者线粒体功能的监测也极为罕见。在本文中,我们描述了过去几年应用于患者的线粒体NADH荧光测量的科学背景和实际用途。除NADH外,我们还从同一组织区域光学测量了微循环血流量和体积,以及HbO(2) 氧合。检测到的四个参数提供了有关组织活力的实时数据,这对于患者的监测至关重要。

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