In this work the Langmuir monolayers were used to prepare multicomponent systems mimicking outer and inner layer of human erythrocyte membranes. The aim of performed experiments was to compare the influence of cholesterol on complex artificial membranes reflecting compositional diversity of the respective membranes leaflets. The properties of both systems in the presence of cholesterol at various concentrations were analyzed by means of thermodynamic description of the interactions between molecules in the investigated monolayers, complemented by the analysis of their morphology performed with the application of Brewster Angle Microcopy. It was found that cholesterol induces highly favorable stabilizing effect on the studied monolayers, increases their condensation and strengthens intermolecular forces. However, the influence of cholesterol was found to be stronger on the mixed film imitating outer membrane layer. Detailed discussion of the obtained results proved that the behavior of cholesterol in the model outer vs. inner layers is strongly determined by their compositional differences. The most important findings resulting from these experiments concern the problem of transbilayer distribution of cholesterol in membranes, which despite numerous investigations seems to be still unclear. The data obtained in this work allow one to suggest that cholesterol tends to accumulate preferentially in outer membrane leaflet or it is localized symmetrically in both layers rather than in inner leaflet.

译文

在这项工作中,Langmuir单层用于制备模仿人红细胞膜外层和内层的多组分系统。进行实验的目的是比较胆固醇对复杂人工膜的影响,反映出相应膜小叶的成分多样性。通过热力学描述所研究的单层中分子之间的相互作用,并通过使用Brewster Angle Microcopy对其形态进行分析,来分析两种系统在各种浓度的胆固醇存在下的特性。发现胆固醇对所研究的单层具有非常有利的稳定作用,增加了它们的凝结并增强了分子间力。然而,发现胆固醇对模仿外膜层的混合膜的影响更强。对获得的结果的详细讨论证明,模型外层与内层中胆固醇的行为很大程度上取决于它们的组成差异。这些实验得出的最重要的发现涉及膜中胆固醇的跨双层分布问题,尽管进行了大量研究,但仍不清楚。在这项工作中获得的数据表明,胆固醇倾向于优先在外膜小叶中积累,或者它对称地定位在两层而不是内小叶中。

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