Dietary restriction (DR) is a robust intervention that extends both health span and life span in many organisms. Ubiquinol and ubiquinone represent the reduced and oxidized forms of coenzyme Q (CoQ). CoQ plays a central role in energy metabolism and functions in several cellular processes including gene expression. Here we used the model organism Caenorhabditis elegans to determine level and redox state of CoQ and expression of genes in response to DR. We found that DR down-regulates the steady-state expression levels of several evolutionary conserved genes (i.e. coq-1) that encode key enzymes of the mevalonate and CoQ-synthesizing pathways. In line with this, DR decreases the levels of total CoQ and ubiquinol. This CoQ-reducing effect of DR is obvious in adult worms but not in L4 larvae and is also evident in the eat-2 mutant, a genetic model of DR. In conclusion, we propose that DR reduces the level of CoQ and ubiquinol via gene expression in the model organism C. elegans.

译文

饮食限制 (DR) 是一种强有力的干预措施,可以延长许多生物的健康寿命和寿命。泛醌和泛醌代表辅酶q (CoQ) 的还原和氧化形式。CoQ在能量代谢中起着核心作用,并在包括基因表达在内的几个细胞过程中发挥作用。在这里,我们使用秀丽隐杆线虫的模式生物来确定CoQ的水平和氧化还原状态以及响应DR的基因表达。我们发现DR下调了几个进化保守基因 (即coq-1) 的稳态表达水平,这些基因编码甲羟戊酸和辅酶q合成途径的关键酶。与此一致,DR降低了总CoQ和泛醇的水平。DR的这种降低CoQ的作用在成年蠕虫中很明显,但在L4幼虫中不明显,在DR的遗传模型eat-2突变体中也很明显。总之,我们建议DR通过模式生物秀丽隐杆线虫中的基因表达降低CoQ和泛醇的水平。

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