The chromosome of R6K contains multiple origins of replication. The origin gamma is infrequently used in the original plasmid and remains "silent" in certain miniplasmid derivatives. The inactivation of the origin is caused by a natural origin silencer located adjacent to the minimal ori gamma sequence. The silencer functions in cis and has no trans activity. It has functional polarity and works only in one orientation when present immediately downstream from ori gamma. The silencer apparently initiates an RNA that invades ori gamma and turns it off either by competing with a primer RNA or by disrupting ori gamma structure. As predicted, removal of the silencer blocks the synthesis of silencer RNA and derepresses the origin.

译文

R6K的染色体包含多个复制起源。原始质粒中很少使用origin γ,在某些小质粒衍生物中保持 “沉默”。原点的失活是由位于最小ori gamma序列附近的自然原点消音器引起的。消音器在顺式中起作用,没有反式活性。它具有功能性极性,并且在ori gamma下游立即存在时仅在一个方向上起作用。消音器显然会启动入侵ori gamma的RNA,并通过与引物RNA竞争或破坏ori gamma结构将其关闭。如预期的那样,去除消音器会阻止消音器RNA的合成并消除原点。

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