We have investigated the relationship between cortisol and dehydroepiandrosterone (DHEA) levels and the immune response to mycobacterial antigens in peripheral venous blood, from a male population of active tuberculosis patients and age-matched healthy controls of the same sex (HCo). Peripheral blood mononuclear cells were cultured for 36 or 96 h with whole sonicated Mycobacterium tuberculosis (WSA) for measurement of proliferation, interferon gamma (IFN-gamma) and interleukin-10 (IL-10) in culture supernatants. Comparisons on the in vitro mycobacterial-driven immune responses demonstrated that TB patients had a higher IL-10 production, a decreased lymphoproliferation and a trend to reduced IFN-gamma synthesis, in relation to HCo. Active disease was also characterized by increases in the plasma levels of glucocorticoids (GC) and reduced concentrations of DHEA which resulted in a higher cortisol/DHEA ratio respect the HCo group. Plasma DHEA levels were positively correlated with IFN-gamma values. An inverse correlation was found between the cortisol/DHEA ratio and IFN-gamma levels. Novel evidence is provided showing that the balance between cortisol and DHEA is partly responsible for the immune perturbations seen in TB patients.

译文

我们已经调查了来自活跃的结核病患者和年龄匹配的同性健康对照 (HCo) 的男性人群的皮质醇和脱氢表雄酮 (DHEA) 水平与外周静脉血中分枝杆菌抗原的免疫反应之间的关系。将外周血单核细胞与全超声结核分枝杆菌 (WSA) 一起培养36或96小时,以测量培养上清液中的增殖,干扰素 γ (IFN-γ) 和interleukin-10 (IL-10)。对体外分枝杆菌驱动的免疫反应的比较表明,与HCo相比,结核病患者具有更高的IL-10产生,淋巴增殖减少以及IFN-γ 合成减少的趋势。活动性疾病的特征还在于血浆糖皮质激素 (GC) 水平升高和DHEA浓度降低,这导致HCo组的皮质醇/DHEA比率更高。血浆DHEA水平与IFN-γ 值呈正相关。皮质醇/DHEA比率与IFN-γ 水平之间呈负相关。提供了新的证据,表明皮质醇和DHEA之间的平衡是结核病患者免疫紊乱的部分原因。

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