We evaluated Trypanosoma cruzi infection in 397 wild Mepraia gajardoi specimens from five coastal localities in northern Chile by detection of minicircle DNA by polymerase chain reaction. The wild capture sites were classified into two ecotopes: a fully wild ecotope (ecotope 1) and a wild ecotope near human dwellings (ecotope 2). Infection rates varied between 11% and 27%. Minicircle hybridization assays showed that T. cruzi lineages Tc II and Tc VI were commonly detected in ecotope 1 and ecotope 2, respectively. These results are discussed in the context of insect proximity to human dwellings, the alimentary profile of Mepraia sp., T. cruzi lineages detected in the past in the same disease-endemic area circulating in humans, and Triatoma infestans.

译文

我们通过聚合酶链反应检测小圆DNA,评估了智利北部五个沿海地区的397个野生Mepraia gajardoi标本中的克氏锥虫感染。野生捕获地点分为两个生态群落: 一个完全野生的生态群落 (ecotope 1) 和一个靠近人类住所的野生生态群落 (ecotope 2)。感染率在11% 和27% 之间变化。小圆杂交试验表明,克氏T。克氏谱系Tc II和Tc VI分别在生态环境1和生态环境2中被普遍检测到。这些结果是在昆虫靠近人类住所,过去在人类中传播的同一疾病流行地区检测到的Mepraia sp。,T. cruzi谱系的饮食特征以及Triatoma infestans的背景下进行讨论的。

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