While growing in the tsetse fly, Trypanosoma brucei expresses a major surface glycoprotein, the procyclic acidic repetitive protein (PARP). The parp genes are transcribed by an alpha-amanitin-resistant RNA polymerase. We have determined the sequence requirements for parp promoter activity. Studies of RNA produced from input DNA in transiently transfected trypanosomes indicate that the RNA is correctly processed by trans-splicing and polyadenylation. Deletion analyses show that 330 bp are sufficient for full promoter and splicing activity and that the promoter structure is complex, involving at least three elements whose mutual spacing is important. Mutagenesis pin-pointed two sequences vital for promoter activity; neither bears any resemblance to known prokaryotic or eukaryotic promoter elements.

译文

在采采蝇中生长时,布氏锥虫表达一种主要的表面糖蛋白,即原环酸性重复蛋白 (PARP)。parp基因由抗 α-amanitin的RNA聚合酶转录。我们已经确定了parp启动子活性的序列要求。对瞬时转染的锥虫中输入DNA产生的RNA的研究表明,通过反式剪接和多聚腺苷酸化可以正确处理RNA。缺失分析表明,330 bp足以实现完整的启动子和剪接活性,并且启动子结构复杂,涉及至少三个相互间隔重要的元素。诱变针尖的两个对启动子活性至关重要的序列; 都没有与已知的原核或真核启动子元件有任何相似之处。

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