Triclosan, triclocarban, 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), and bisphenol A (BPA) have been reported to disturb thyroid hormone (TH) homeostasis. We have examined the effects of these chemicals on sodium/iodide symporter (NIS)-mediated iodide uptake and the expression of genes involved in TH synthesis in rat thyroid follicular FRTL-5 cells, and on the activity of thyroid peroxidase (TPO) using rat thyroid microsomes. All four chemicals inhibited NIS-mediated iodide uptake in a concentration-dependent manner. A decrease in the iodide uptake was also observed in the absence of sodium iodide. Kinetic studies showed that all four chemicals were non-competitive inhibitors of NIS, with the order of Ki values being triclosan300 μM, respectively. Neither BDE-47 nor BPA affected TPO activity. In conclusion, triclosan, triclocarban, BDE-47, and BPA inhibited iodide uptake, but had differential effects on the expression of TH synthesis-related genes and the activity of TPO.

译文

据报道,三氯生,三氯卡班,2,2 ',4,4'-四溴二苯醚 (BDE-47) 和双酚a (BPA) 会干扰甲状腺激素 (TH) 的稳态。我们已经使用大鼠甲状腺微粒体研究了这些化学物质对钠/碘同向转运体 (NIS) 介导的碘化物摄取和参与TH合成的基因表达的影响,以及对甲状腺过氧化物酶 (TPO) 活性的影响。所有四种化学物质均以浓度依赖性方式抑制NIS介导的碘化物吸收。在没有碘化钠的情况下,还观察到碘化物的吸收减少。动力学研究表明,所有四种化学物质都是NIS的非竞争性抑制剂,Ki值的顺序为三氯生 <三氯卡班 300 μ m时抑制TPO的活性。BDE-47和BPA均不影响TPO活性。总之,三氯生,三氯卡班,BDE-47和BPA抑制碘化物的吸收,但对TH合成相关基因的表达和TPO的活性有不同的影响。

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