Two strains of Aeromonas salmonicida, YK and BG, were isolated from largemouth bronze gudgeon and northern whitefish in China, and identified as A. salmonicida subsp. salmonicida based on phylogenetic analysis of vapA and 16S rRNA gene sequences. YK and BG originated from freshwater fish, one of which belonged to the cyprinid family, and the strains showed a difference in virulence. Subsequently, we performed whole genome sequencing of the strains, and comparison of their genomic sequences to the genome of the A449 reference strain revealed various genomic rearrangements, including a new variant of the genomic island AsaGEI in BG, designated as AsaGEI2c This is the first report on a GEI of A. salmonicida strain from China. Furthermore, both YK and BG strains contained a Tn7 transposon inserted at the same position in the chromosome. Finally, IS-dependent rearrangements on pAsa5 are deemed likely to have occurred, with omission of the resD gene in both strains as well as omission of genes related to the IncF conjugal transfer system in the YK isolate. This study demonstrates that A. salmonicida subsp. salmonicida can infect non-salmonids (cyprinids) in addition to salmonids, and that AsaGEI2c might be useful as a geographical indicator of Chinese A. salmonicida subsp. salmonicida isolates.

译文

从中国大嘴青铜鱼和北白鲑中分离出两株沙门氏菌气单胞菌YK和BG,并鉴定为沙门氏菌亚种。基于vapA和16S rRNA基因序列的系统发育分析的沙门氏菌。YK和BG起源于淡水鱼,其中一种属于鲤科,菌株表现出毒力差异。随后,我们对菌株进行了全基因组测序,并将其基因组序列与A449参考菌株的基因组进行比较,揭示了各种基因组重排,包括BG中的基因组岛AsaGEI的新变体,被指定为AsaGEI2c这是来自中国的沙门氏菌菌株GEI的第一份报告。此外,YK和BG菌株均包含插入染色体相同位置的Tn7转座子。最后,认为pAsa5上的IS依赖性重排可能已经发生,两个菌株中resD基因均被省略,并且YK分离株中与IncF结合转移系统相关的基因也被省略。这项研究表明,沙门氏菌亚种。除鲑鱼外,鲑鱼还可以感染非鲑鱼 (鲤科鱼),而AsaGEI2c可能用作中国鲑鱼亚种的地理指标。鲑鱼分离株。

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