Poly(1-vinyl-2-pyrrolidinone) (PVP) and copolymers of 1-vinyl-2-pyrrolidinone are insoluble in water when crosslinked but they can absorb very large amounts of water to become syringe-injectable hydrogels. Such gels have been investigated recently as potential substitutes for the vitreous humour in the eye. In this study, during the cytotoxic evaluation by sulforhodamine B colorimetric assay of variously crosslinked PVP gels, it was found that many of them showed protective/growth promoting effects on 3T3 mouse fibroblasts in static cultures, a phenomenon encountered previously only with aqueous solutions of a limited number of natural or synthetic polymers. Particularly, the gels crosslinked with diethylene glycol dimethacrylate (DEGDMA) induced a significant enhancement of cell proliferation, especially in serum-free cultures. No correlation between this effect and the essential gel properties (chemical composition, viscoelasticity and equilibrium water content) could be established. The study demonstrated that crosslinked PVP hydrogels showed a serum-like growth promoting effect on an anchorage-dependent cell line, which may be due to physical protection, inability of the insoluble gels to penetrate cell membranes, and their ability to mimic the extracellular matrix.

译文

聚 (1-乙烯基-2-吡咯烷酮) (PVP) 和1-乙烯基-2-吡咯烷酮的共聚物在交联时不溶于水,但它们可以吸收大量的水,成为可注射器注射的水凝胶。最近已经研究了这种凝胶作为眼睛玻璃体液的潜在替代品。在这项研究中,在通过sulforhodamine B比色法对各种交联的PVP凝胶进行细胞毒性评估期间,发现其中许多凝胶对静态培养中的3T3小鼠成纤维细胞表现出保护/促进生长的作用,这种现象以前仅在水溶液中遇到。有限数量的天然或合成聚合物。特别是,与二甘醇二甲基丙烯酸酯 (DEGDMA) 交联的凝胶可显着增强细胞增殖,尤其是在无血清培养物中。无法建立这种作用与基本凝胶特性 (化学成分,粘弹性和平衡水含量) 之间的相关性。研究表明,交联的PVP水凝胶对锚定依赖性细胞系表现出类似血清的生长促进作用,这可能是由于物理保护,不溶性凝胶无法穿透细胞膜以及它们模仿细胞外基质的能力。

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