Electrical fields are known to interact with human cells. This principle has been explored to regulate cellular activities for bone tissue regeneration. In this work, Saos-2 cells were cultured on conductive scaffolds made of biodegradable poly(L-lactide) and the heparin-containing, electrically conducting polypyrrole (PPy/HE) to study their reaction to electrical stimulation (ES) mediated through such scaffolds. Both the duration and intensity of ES enhanced cell proliferation, generating a unique electrical intensity and temporal "window" within which osteoblast proliferation was upmodulated in contrast to the downmodulation or ineffectiveness in other ES regions. The favourable ES intensity (200 mV/mm) was further investigated in terms of the gene activation and protein production of two important osteoblast markers characterised by extracellular matrix maturation and mineralisation, that is alkaline phosphatase (ALP) and osteocalcin (OC). Both genes were found activated and the relevant protein production increased significantly following ES. In contrast, ES in the down-modulation region (400 mV/mm) suppressed the production of both ALP and OC. This work demonstrated that important osteoblast markers can be modulated with specific ES parameters mediated through conductive polymer substrates, providing a unique strategy for bone tissue engineering.

译文

已知电场与人类细胞相互作用。已探索此原理来调节骨组织再生的细胞活性。在这项工作中,将Saos-2细胞培养在由可生物降解的聚 (L-丙交酯) 和含肝素的导电聚吡咯 (PPy/HE) 制成的导电支架上,以研究它们对通过此类支架介导的电刺激 (ES) 的反应。ES的持续时间和强度都增强了细胞增殖,产生了独特的电强度和时间 “窗口”,与其他ES区域的下调或无效相反,在该窗口内对成骨细胞增殖进行了上调。进一步研究了有利的ES强度 (200  mV/mm),即两种重要的成骨细胞标志物的基因活化和蛋白质生产,其特征是细胞外基质成熟和矿化,即碱性磷酸酶 (ALP) 和骨钙素 (OC)。发现两个基因都被激活,并且在ES之后相关的蛋白质产量显着增加。相反,下调制区域 (400  mV/mm) 中的ES抑制了ALP和OC的产生。这项工作表明,重要的成骨细胞标志物可以通过导电聚合物底物介导的特定ES参数进行调节,为骨组织工程提供了独特的策略。

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