Magnetic-activated cell sorting (MACS) using paramagnetic annexin V-conjugated microbeads eliminates spermatozoa with externalized phosphatidylserine, which is considered one of the features of apoptosis. The objective of this study was to evaluate sperm recovery following the use of MACS as a sperm preparation technique. Mature spermatozoa were separated and divided into two fractions: the first was prepared by density gradient centrifugation (DGC) and MACS, while the second was prepared by DGC only. Following MACS, the percentage of cells collected in the annexin-negative fraction was significantly higher than the annexin-positive fraction and the sperm recovery rate was 73.8 +/- 12.1%. In conclusion, the integration of MACS with DGC can be considered as an effective sperm preparation technique that does not lead to significant cell loss. Separating a distinctive population of non-apoptotic spermatozoa with intact membranes may optimize the outcome of assisted reproduction.

译文

使用顺磁性膜联蛋白V偶联微珠的磁激活细胞分选 (MACS) 消除了具有外化磷脂酰丝氨酸的精子,这被认为是凋亡的特征之一。这项研究的目的是评估使用MACS作为精子制备技术后的精子恢复情况。将成熟的精子分离并分为两个部分: 第一个部分通过密度梯度离心 (DGC) 和MACS制备,而第二个部分仅通过DGC制备。在MACS之后,在膜联蛋白阴性部分中收集的细胞百分比显著高于膜联蛋白阳性部分,并且精子治愈率为73.8 +/- 12.1%。总之,MACS与DGC的整合可以被认为是一种有效的精子制备技术,不会导致细胞大量丢失。分离具有完整膜的独特的非凋亡精子群体可能会优化辅助生殖的结果。

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