Plant-mediated RNAi has been developed as a powerful weapon in the fight against agricultural insect pests. The gap gene hunchback (hb) is of crucial importance in insect axial patterning and knockdown of hb is deforming and lethal to the next generation. The peach potato aphid, Myzus persicae (Sulzer), has many host plants and can be found throughout the world. To investigate the effect of plant-mediated RNAi on control of this insect, the hb gene in M. persicae was cloned, plant RNAi vector was constructed, and transgenic tobacco expressing Mphb dsRNA was developed. Transgenic tobacco had a different integration pattern of the transgene. Bioassays were performed by applying neonate aphids to homozygous transgenic plants in the T2 generation. Results revealed that continuous feeding of transgenic diet reduced Mphb mRNA level in the fed aphids and inhibited insect reproduction, indicating successful knockdown of the target gene in M. persicae by plant-mediated RNAi.

译文

植物介导的RNAi已被开发为对抗农业害虫的有力武器。gap基因驼背 (hb) 在昆虫轴向图案化中至关重要,并且hb的敲除正在变形并致死下一代。桃马铃薯蚜虫Myzus persicae (Sulzer) 具有许多寄主植物,在世界各地都可以找到。为了研究植物介导的RNAi对该昆虫的控制作用,克隆了桃蚜中的hb基因,构建了植物RNAi载体,并开发了表达Mphb dsRNA的转基因烟草。转基因烟草具有不同的转基因整合模式。通过将新生蚜虫应用于T2代的纯合转基因植物进行生物测定。结果表明,连续饲喂转基因饮食可降低饲喂蚜虫中的Mphb mRNA水平并抑制昆虫繁殖,表明植物介导的RNAi成功敲除了桃蚜中的靶基因。

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