In a study of 55 patients with either acute lymphoid leukemia (ALL; 25 cases) or acute myeloid leukemia (AML; 30 cases), paraffin-embedded bone marrow particle sections were examined with a panel of monoclonal and polyclonal antibodies reactive toward lymphoid and myeloid-associated antigens, using the alkaline phosphatase-anti-alkaline phosphatase (APAAP) technique. All cases were previously classified according to the French-American-British (FAB) Co-operative Group, and cases of ALL were immunophenotyped by flow cytometry. Results indicated that myeloid-associated antibodies (Mac 387, KP 1 [CD68], antielastase, antilactoferrin, and antilysozyme) did not react with any case of ALL, M1-AML, or M6-AML, whereas at least one of these antibodies reacted with 20 of 21 (95%) cases of M2, M3, M4, and M5-AML. Anti-glycophorin C marked cases of M6-AML, whereas anti-CD3 labeled T-cell ALL. None of the antibodies tested specifically identified cases of B-cell ALL. The authors conclude that use of a selected panel of antibodies on paraffin-embedded bone marrow particle sections may be of value in the diagnosis and immunophenotypic classification of many cases of acute leukemias.

译文

在对55例急性淋巴白血病 (ALL; 25例) 或急性髓细胞性白血病 (AML; 30例) 患者的研究中,用一组对淋巴样和髓样相关抗原具有反应性的单克隆和多克隆抗体检查了石蜡包埋的骨髓颗粒切片,使用碱性磷酸酶-抗碱性磷酸酶 (APAAP) 技术。所有病例先前均按法美英 (FAB) 合作组分类,所有病例均通过流式细胞术进行免疫表型。结果表明,髓样相关抗体 (Mac 387,KP 1 [CD68],抗弹性蛋白酶,抗乳铁蛋白和抗溶菌酶) 未与ALL,M1-AML或M6-AML的任何病例发生反应,而这些抗体中的至少一种与21例 (95% 例) M2,M3,m4和M5-AML。抗糖蛋白C标记了M6-AML病例,而anti-CD3标记了T细胞ALL。测试的抗体均未特异性鉴定出b细胞ALL病例。作者得出的结论是,在石蜡包埋的骨髓颗粒切片上使用选定的一组抗体可能对许多急性白血病病例的诊断和免疫表型分类具有价值。

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