Reconstructing segmental costal cartilage defects resulting from autologous cartilage grafts in plastic surgery remains a challenge. The present study focused on a biomimetic strategy for in situ costal cartilage regeneration that did not rely on an autogenous/xenogenous tissue graft. A multifunctional biomimetic SGII/HA-DN hydrogel based on a "chemical-curing, shaping, and light-curing" gelation system was developed and evaluated for its mechanical properties, clinical applications and biological functions. This hydrogel showed good suitability to repair defects and a high mechanical support strength (11 MPa, which is close to the natural strength of costal cartilage). Biologically, the hydrogel exhibited dual-immunomodulatory effects on the pro-inflammatory/anti-inflammatory phenotypes of neutrophils and M1/M2 macrophage polarization and subsequently promoted the chondrogenesis of cartilage stem/progenitor cells through both direct induction and indirect stimulation by the M2 macrophage-mediated TGF-β/Smad pathway. Furthermore, this SGII/HA-DN hydrogel could regulate the local microenvironment, inducing new costal cartilage regeneration in vivo. Our findings demonstrate that the newly developed multifunctional SGII/HA-DN hydrogel provides a strategy with high prospect for the biomimetic repair of segmental costal cartilage defects in clinical practice.

译文

在整形外科中重建自体软骨移植物导致的节段性肋软骨缺损仍然是一个挑战。本研究的重点是不依赖于自体/异种组织移植物的原位肋软骨再生的仿生策略。开发了一种基于 “化学固化,整形和光固化” 凝胶系统的多功能仿生SGII/HA-DN水凝胶,并对其机械性能,临床应用和生物学功能进行了评估。该水凝胶显示出良好的修复缺陷的适用性和高的机械支撑强度 (11 MPa,接近肋软骨的自然强度)。生物学上,水凝胶对中性粒细胞的促炎/抗炎表型和M1/M2巨噬细胞极化表现出双重免疫调节作用,随后通过M2巨噬细胞介导的TGF-β/Smad途径的直接诱导和间接刺激促进软骨干/祖细胞的软骨形成。此外,该SGII/HA-DN水凝胶可以调节局部微环境,在体内诱导新的肋软骨再生。我们的发现表明,新开发的多功能SGII/HA-DN水凝胶为临床实践中节段性肋软骨缺损的仿生修复提供了一种前景广阔的策略。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录