The present study is aimed to identify the prevalence of Blastocystis subtypes isolated from patients in a major hospital in northeastern Thailand. A total of 562 stool samples were examined by culture technique, and 56 Blastocystis-positive samples were analyzed further by the combination of restriction fragment length polymorphism (RFLP) followed by polymerase chain reaction with sequence-tagged site primers (PCR-STS). By RFLP profiles, Blastocystis genotypes were categorized into four groups: group A (12, 21.4%), group B (32, 57.1%), group C (10, 17.9%), and group D (2, 3.6%). By PCR-STS, only four subtypes were identified. All 12 (21.4%) isolates in group A were identified as subtype 1. Similarly, all 32 (57.1%) isolates in group B were subtype 3. In group C, 10 (17.9%) samples were all subtype 7, and two samples (3.6%) in group D were both subtype 6. Of 56 Blastocystis-positive patients, 31 (55.4%) were asymptomatic and 22 (39.4%) have gastrointestinal symptoms. No significant association was observed between the Blastocystis subtypes and the clinical features. Among the Blastocystis-positive patients, the most characteristic stool samples were loose (78.6%) and soft (17.9%). In conclusion, the most common Blastocystis spp. in northeastern Thailand was subtype 3 followed by subtype 1. Relatively minor subtypes, subtype 6 and subtype 7 which are considered as avian subtypes, were found for the first time in humans in Thailand.

译文

本研究旨在确定从泰国东北部一家主要医院的患者中分离出的囊胚亚型的患病率。通过培养技术共检查了562份粪便样品,并通过结合限制性片段长度多态性 (RFLP),然后通过聚合酶链式反应与序列标记的位点引物 (pcr-sts) 进一步分析了56份囊胚阳性样品。根据RFLP图谱,囊胚基因型分为四组: A组 (12、21.4%),B组 (32、57.1%),C组 (10、17.9%) 和D组 (2、3.6%)。通过pcr-sts,仅鉴定出四种亚型。组A中的所有12个 (21.4%) 分离株均鉴定为亚型1。类似地,组B中的所有32 (57.1%) 分离株均为亚型3。C组10个 (17.9% 个) 样本均为亚型7,D组2个样本 (3.6% 个) 均为亚型6。在56例囊胚阳性患者中,31例 (55.4% 例) 无症状,22例 (39.4% 例) 有胃肠道症状。未观察到囊胚亚型与临床特征之间的显着关联。在囊胚阳性患者中,最典型的粪便样本为疏松 (78.6%) 和柔软 (17.9%)。总之,最常见的胚泡藻属。在泰国东北部,亚型为3,其次是亚型1。在泰国的人类中首次发现了相对较小的亚型,即被认为是鸟类亚型的亚型6和亚型7。

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