Opioid receptor binding was evaluated in parahippocampal cortex (PHC) obtained from patients with intractable mesial temporal lobe epilepsy (MTLE) with and without subacute high frequency electrical stimulation (HFS) in this brain area. Mu, delta and nociceptin receptor binding was determined by autoradiography in PHC of five patients (ESAE group) with MTLE history of 14.8 +/- 2.5 years and seizure frequency of 11 +/- 2.9 per month, two of them (40%) with mesial sclerosis. This group demonstrated antiepileptic effects following subacute HFS (130 Hz, 450 micros, 200-400 microA), applied continuously during 16-20 days in PHC. Values were compared with those obtained from patients with severe MTLE (history of 21.7 +/- 2.8 years and seizure frequency of 28.2 +/- 14 per month) in whom electrical stimulation did not induce antiepileptic effects (ESWAE group, n = 4), patients with MTLE in whom no electrical stimulation was applied (MTLE group, n = 4) and autopsy material acquired from subjects without epilepsy (n = 4 obtained from three subjects). Enhanced 3H-DAMGO (MTLE, 755%; ESAE, 375%; ESWAE, 693%), 3H-DPDPE (MTLE, 242%; ESAE, 80%; ESWAE, 346%) and 3H-nociceptin (MTLE, 424%; ESAE, 217%; ESWAE, 451%) binding was detected in the PHC of all epileptic groups. However, tissue obtained from ESAE group demonstrated lower opioid receptor binding (3H-DAMGO, 44.5%, p < 0.05; 3H-DPDPE, 47%, p < 0.05; 3H-nociceptin, 39.3%, p < 0.5) when compared with MTLE group. The present results indicate that a high effectiveness to the antiepileptic effects induced by HFS is associated with reduced opioid peptide binding.

译文

在患有或没有亚急性高频电刺激 (HFS) 的难治性颞叶内侧颞叶癫痫 (MTLE) 患者的海马旁皮层 (PHC) 中评估了阿片样物质受体的结合。通过放射自显影法测定了5例 (ESAE组) MTLE病史为14.8/- 2.5年,每月发作频率为11//- 2.9的PHC患者的Mu,delta和伤害肽受体的结合,其中2例 (40%) 患有内侧硬化。该组在亚急性HFS (130Hz,450微子,200-400微子) 在PHC中连续施加16-20天后表现出抗癫痫作用。将电刺激未引起抗癫痫作用的重度MTLE患者 (21.7 +/- 2.8年病史,每月发作频率为28.2 +/- 14次) 的值进行比较 (ESWAE组,n = 4),未施加电刺激的MTLE患者 (MTLE组,n = 4) 和从无癫痫的受试者获得的尸检材料 (n = 4)。在所有癫痫组的PHC中检测到增强的3H-DAMGO (MTLE,755%; ESAE,375%; ESWAE,693%) 、3H-DPDPE (MTLE,242%; ESAE,80%; ESWAE,346%) 和3H-nociceptin (MTLE,424%; ESAE,217%; ESWAE,451%) 结合。然而,与MTLE组相比,从ESAE组获得的组织显示出较低的阿片样物质受体结合 (3H-DAMGO,44.5%,p <0.05; 3H-DPDPE,47%,p <0.05; 3H-nociceptin,39.3%,p <0.5)。目前的结果表明,HFS诱导的抗癫痫作用的高有效性与阿片肽结合减少有关。

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