Cardiac troponin T (cTnT) and troponin I (cTnI) have been suggested as new, more specific markers of myocardial cellular damage. The objective of this study was to examine how the distributions of cTnI and cTnT were affected in postinfarction left ventricular remodeled (LVR) myocardium. At 2 months postinfarct in a porcine heart failure model, both Western blot and biochemical assay analyses were performed on left ventricular myocardium remote from the infarct zone in ligation animals (n = 8). Results were compared with data from the left ventricular myocardium from similar sized healthy (control) pigs (n = 7). Autoradiograms from Western blot analysis showed that the protein mass for cTnI and cTnT in LVR hearts decreased 80% (P < 0.001) and 40% (P < 0.02), respectively, when compared with nondiseased tissue. Similarly, the concentrations for cTnI and cTnT in LVR hearts decreased 42% (P < 0.05) and 70% (P < 0.001), respectively, compared with nondiseased normal tissue. The clinical assumption is that the appearance of cTnI and cTnT in the blood is proportional to chronic loss of cTnI and cTnT from injured myocardium associated with left ventricular remodeling.

译文

心肌肌钙蛋白T (cTnT) 和肌钙蛋白I (cTnI) 已被认为是新的,更特异的心肌细胞损伤标志物。这项研究的目的是研究梗死后左心室重塑 (LVR) 心肌中cTnI和cTnT的分布如何受到影响。在猪心力衰竭模型中,在梗塞后2个月,对结扎动物 (n = 8) 远离梗塞区的左心室心肌进行了Western印迹和生化测定分析。将结果与类似大小的健康 (对照) 猪 (n = 7) 的左心室心肌数据进行了比较。来自Western blot分析的放射自显影图显示,与未患病组织相比,LVR心脏中cTnI和cTnT的蛋白质质量分别降低了80% (P < 0.001) 和40% (P <0.02)。同样,与未患病的正常组织相比,LVR心脏中cTnI和cTnT的浓度分别降低了42% (P < 0.05) 和70% (P <0.001)。临床假设是血液中cTnI和cTnT的出现与与左心室重构相关的受损心肌的cTnI和cTnT的慢性损失成正比。

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