In a previous large scale screen for differentially expressed genes in pancreatic cancer, we identified a gene highly overexpressed in cancer encoding a novel protein with four K-homologous (KH) domains. KH-domains are found in a subset of RNA-binding proteins, including pre-mRNA-binding (hnRNP) K protein and the fragile X mental retardation gene product (FMR1). By fluorescence in situ hybridization (FISH) the identified gene named koc (KH domain containing protein overexpressed in cancer) was assigned to chromosome 7p11.5. Two pseudogenes were localised on chromosome 6 and 11. The cloned koc cDNA has a 250 bp 5'-UTR, a 1740 bp ORF and a 2168 bp 3'-UTR. The AU-rich 3'-untranslated region of koc contains eight AUUUA and four AUUUUUA reiterated motifs. The deduced koc protein with 580 amino-acids has a relative molecular mass (Mr) of approximately 65,000 (65 K). The koc transcript is highly overexpressed in pancreatic cancer cell lines and in pancreatic cancer tissue as compared to both, normal pancreas and chronic pancreatitis tissue. High levels of expression were as well found in tissue samples of other human tumours. As the KH domain has been shown to be involved in the regulation of RNA synthesis and metabolism, we speculate that koc may assume a role in the regulation of tumour cell proliferation by interfering with transcriptional and or posttranscriptional processes. However, the precise role of koc in human tumour cells is unknown and remains to be elucidated.

译文

在先前对胰腺癌中差异表达基因的大规模筛选中,我们鉴定了一个在癌症中高度过表达的基因,该基因编码具有四个K同源 (KH) 域的新型蛋白质。KH结构域存在于RNA结合蛋白的子集中,包括前mRNA结合 (hnRNP) K蛋白和脆弱的X智力低下基因产物 (FMR1)。通过荧光原位杂交 (FISH),将鉴定出的名为koc (含KH结构域的蛋白质在癌症中过表达) 的基因分配到7p11.5号染色体上。两个假基因位于6号和11号染色体上。克隆的koc cDNA具有250 bp 5 '-UTR、1740 bp ORF和2168 bp 3'-UTR。科威特石油公司富含非盟的3 '-非翻译区包含八个AUUUA和四个auuuuuua重申的图案。推导的具有580个氨基酸的koc蛋白具有约65,000 (65 K) 的相对分子质量 (Mr)。与正常胰腺和慢性胰腺炎组织相比,koc转录本在胰腺癌细胞系和胰腺癌组织中高度过表达。在其他人类肿瘤的组织样本中也发现了高水平的表达。由于KH结构域已被证明参与RNA合成和代谢的调节,因此我们推测koc可能通过干扰转录和或转录后过程而在肿瘤细胞增殖的调节中发挥作用。然而,koc在人类肿瘤细胞中的确切作用尚不清楚,尚待阐明。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录