This is the first study to explore the effect of biomaterial on tooth germ cell adhesion and proliferation in vitro. The purpose of this study is to evaluate the effects of cell-surface interactions of tooth germ cells on biomaterials with various surface hydrophilicities. The biomaterials used in this study included polyvinyl alcohol (PVA), poly(lactic-co-glycolic acid) (PLGA), poly(ethylene-co-vinyl alcohol; EVAL), and polyvinylidene fluoride (PVDF). Cell morphology was observed by photomicroscopy. Cell growth was assayed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction activity and the characteristic expression of amelogenin and collagen type I in tooth germ cells was investigated using immunocytochemistry. The results indicated that adhesion and proliferation of tooth germ cells to biomaterials with moderate hydrophilicity/hydrophobicity was superior compared to most hydrophobic material PVDF or mosthydrophilic material PVA in this study. Cellular adhesion and proliferation was evident on all tested biomaterials except PVA. The cell spheroids on PVA appeared not to be proliferated and remained as well as reattachable to tissue culture plates. In conclusion, biomaterials with moderate hydrophilicity are suitable for adhesion and proliferation of tooth germ cells. The material PVA may be a good biomaterial for maintaining tooth germ cells in three-dimensional biological restoration.

译文

这是首次探索生物材料对体外牙胚细胞粘附和增殖的影响的研究。这项研究的目的是评估牙胚细胞的细胞-表面相互作用对具有各种表面亲水性的生物材料的影响。本研究中使用的生物材料包括聚乙烯醇 (PVA),聚 (乳酸-乙醇酸) (PLGA),聚 (乙烯-乙烯醇; EVAL) 和聚偏二氟乙烯 (PVDF)。通过显微显微镜观察细胞形态。用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑鎓 (MTT) 还原活性测定细胞生长,并使用免疫细胞化学研究牙胚细胞中釉原蛋白和I型胶原的特征性表达。结果表明,与大多数疏水性材料PVDF或嗜性材料PVA相比,牙胚细胞对具有中等亲水性/疏水性的生物材料的粘附和增殖要好。除PVA外,所有测试的生物材料都有明显的细胞粘附和增殖。PVA上的细胞球体似乎没有增殖,并且仍然可以重新附着到组织培养板上。总之,具有中等亲水性的生物材料适用于牙胚细胞的粘附和增殖。材料PVA可能是在三维生物修复中维持牙胚细胞的良好生物材料。

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