High-fat-feeding in rats has been reported to enhance stress reactions, as assessed by elevation of blood glucose and corticosterone levels. This study was designed to investigate the relationship between changes in blood glucose and hypothalamic neuronal noradrenaline activity (HNNA), as indexed by the ratio of dihydroxyphenylethyleneglycol (DHPG) to noradrenaline (NA), following physiological stress in high-fat-fed rats. Two groups of adult male Wistar rats were fed isocaloric diets high in fat (59% of calories) or starch (70% of calories). After 3 weeks each of these groups was further subdivided into (a) control, (b) 2 min ambient temperature (20 degrees C) swim or (c) 2 min swim in ice-cold water. Animals were decapitated 20 min after commencing the swim; trunk blood and a sample of medial basal hypothalamus were obtained. Computerized gas chromatography/mass spectrometry was used to measure hypothalamic DHPG and NA concentrations. There were no differences between fat- and starch-fed rats in basal levels of serum glucose, insulin or corticosterone and no differences in DHPG, NA or DHPG/NA ratio. Compared to starch-fed rats, ambient swim stress in the fat-fed group produced significantly larger serum glucose (P less than 0.01), serum corticosterone (P less than 0.05), DHPG (P less than 0.05) and DHPG/NA (P less than 0.01) responses. Following cold swim stress similar differences between fat- and starch-fed animals were observed. In addition, serum insulin was found to be significantly suppressed in the fat-fed group (P less than 0.05) following cold swim.(ABSTRACT TRUNCATED AT 250 WORDS)

译文

据报道,通过血糖和皮质酮水平升高来评估,大鼠高脂喂养可增强应激反应。这项研究旨在研究高脂喂养的大鼠在生理应激后,血糖变化与下丘脑神经元去甲肾上腺素活性 (HNNA) 之间的关系,该变化以二羟基苯乙醇酯 (DHPG) 与去甲肾上腺素 (NA) 的比率为指标。给两组成年雄性Wistar大鼠喂食高脂肪 (59% 卡路里) 或淀粉 (70% 卡路里) 的等热量饮食。3周后,这些组中的每一个进一步细分为 (a) 对照,(b) 环境温度2分钟 (20摄氏度) 游泳或 (C) 在冰冷的水中游泳2分钟。游泳后20分钟将动物斩首; 获得躯干血液和内侧基底下丘脑样本。使用计算机气相色谱/质谱法测量下丘脑DHPG和NA浓度。在脂肪喂养和淀粉喂养的大鼠之间,血清葡萄糖,胰岛素或皮质酮的基础水平没有差异,而DHPG,NA或DHPG/NA比率也没有差异。与淀粉喂养的大鼠相比,脂肪喂养组的环境游泳应激产生明显更大的血清葡萄糖 (P小于0.01),血清皮质酮 (P小于0.05),DHPG (P小于0.05) 和DHPG/NA (P小于0.01) 反应。在冷游泳压力后,观察到以脂肪和淀粉喂养的动物之间存在相似的差异。此外,在冷游泳后,发现脂肪喂养组的血清胰岛素被显着抑制 (P小于0.05)。(摘要截短于250字)

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