In a search for iron-regulated proteins of Aspergillus nidulans and Aspergillus fumigatus a 16-kDa protein was identified which is about 5-fold upregulated during iron starvation in both species and which can be approximately 500-fold enriched by simple one-step chromatography on Amberlite XAD-16 resin. N-terminal protein sequence analysis and cloning of the respective A. nidulans cDNA identified this protein as a Cu/Zn-superoxide dismutase (SODA). Northern analysis revealed that upregulation of sodA expression occurs at the level of transcript accumulation. This seems to be a specific low iron response and not a general starvation answer since sodA transcript levels do not respond to carbon or nitrogen starvation. In contrast, copper depletion leads to transcriptional downregulation of sodA. Furthermore, sodA expression was found still to be subject to iron regulation in an A. nidulans mutant lacking SREA, a regulator of iron homeostasis, indicating that sodA expression is regulated by an SREA-independent mechanism. The data presented suggest that SODA plays a protective role under iron deplete conditions.

译文

在寻找构巢曲霉和烟曲霉的铁调节蛋白时,鉴定出一种16 kDa蛋白,该蛋白在两种物种的铁饥饿期间上调约5倍,并且可以通过简单的一步色谱法富集约500倍在Amberlite XAD-16树脂上。N末端蛋白序列分析和相应的A.Niddulans cDNA的克隆将该蛋白鉴定为Cu/Zn-超氧化物歧化酶 (SODA)。Northern分析表明,苏打表达的上调发生在转录本积累的水平上。这似乎是一种特定的低铁反应,而不是一般的饥饿答案,因为苏打水转录水平对碳或氮的饥饿没有反应。相反,铜的耗竭导致苏打的转录下调。此外,在缺乏铁稳态调节剂SREA的A.Niddulans突变体中,发现苏打表达仍受铁调节,这表明苏打表达受SREA独立机制的调节。提供的数据表明,苏打在铁耗尽条件下起着保护作用。

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