Isolation of phenolic diterpene constituents from the freeze-dried leaves of Rosmarinus officinalis has been obtained by supercritical extraction with carbon dioxide. To determine the ideal conditions for the maximum yield of extract, nine different conditions using three levels of pressures (3000, 4000 and 5000 psi) in combination with three temperatures at 40, 60 and 80 degrees C, respectively, in combination with the analyses of the corresponding antioxidant activities and constituents which existed in extracts has been investigated. The antioxidant activity of each obtained extract was determined by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals test. GC/MS method was used as an alternative to conventional HPLC method for the determination of the principal antioxidant constituents in extract, including phenolic diterpenes carnosic acid (CA) and carnosol (CAL). The confirmation of CA and CAL in extract was forward performed by subjecting HPLC isolates from extract into an ion trap mass spectrometer through an electrospray ionization (ESI) interface for MS/MS analysis. These results indicate that an ideal extraction process was obtained at 5000 psi and 80 degrees C with an extraction yield of 4.27% (w/w) and rich in phenolic antioxidants CA and CAL as contents of 35.23 and 0.46 mg g(-1) in extract, respectively.

译文

通过二氧化碳超临界萃取,从迷迭香冻干叶片隔离出酚二萜成分。为了确定提取物最大产量的理想条件,使用三种压力水平 (3000、4000和5000 psi) 分别在40、60和80 ℃ 下结合三种温度的九种不同条件,结合对提取物中存在的相应抗氧化活性和成分的分析进行了研究。通过使用2,2-二苯基-1-吡啶肼基 (DPPH) 自由基测试来确定每种获得的提取物的抗氧化活性。GC/MS方法被用作常规HPLC方法的替代方法,用于测定提取物中的主要抗氧化剂成分,包括酚二萜肌酸 (CA) 和肌醇 (CAL)。通过将提取物中的HPLC分离物通过电喷雾电离 (ESI) 界面进入离子阱质谱仪进行MS/MS分析,从而对提取物中的CA和CAL进行确认。这些结果表明,在5000 psi和80 ℃ 下获得了理想的提取工艺,提取率为4.27% (w/w),提取物中富含酚类抗氧化剂CA和CAL的含量分别为35.23和0.46 mg g(-1)。

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