In order to illustrate the underlining mechanism of the effect of high pressure on lipases from different resources, the influence of compressed carbon dioxide treatment on the esterification activities and conformation of the three lipases Candida rugosa lipase (CRL), Pseudomonas fluorescens lipase, and Rhizopus oryzae lipase was investigated in the present work. The results showed that the lipases activities were significantly enhanced in most of high-pressure treatments, except the pressure had a negative effect on CRL activity in supercritical condition. Mild depressurization rate could remain the lipase's activity by protecting its rigid structure under supercritical fluid. Conformational analysis by Fourier transform-infrared spectrometry and fluorescence emission spectra revealed that the variances of lipase activity after high-pressure treatment were correlated with the changes of its α-helix content and fluorescence intensity. Additionally, transesterification catalyzed by three lipases in supercritical carbon dioxide were conducted, and 87.2 % biodiesel conversion was obtained by CRL after 3 h, resulting in a great reduction of reaction time.

译文

为了说明高压对不同资源脂肪酶影响的机理,压缩二氧化碳处理对三种脂肪酶酯化活性和构象的影响在本工作中进行了研究。结果表明,在大多数高压处理中,脂肪酶的活性显着提高,但在超临界条件下,压力对CRL活性有负面影响。温和的降压速率可以通过在超临界流体下保护脂肪酶的刚性结构来保持脂肪酶的活性。傅里叶变换红外光谱和荧光发射光谱的构象分析表明,高压处理后脂肪酶活性的变化与其 α-螺旋含量和荧光强度的变化有关。此外,在超临界二氧化碳中进行了由三种脂肪酶催化的酯交换反应,并在3小时后通过CRL获得了87.2% 的生物柴油转化,从而大大减少了反应时间。

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