A novel phytoplasma subgroup strain SC-Phy385 (GenBank accession number HF586648) was found associated with Sugarcane Grassy Shoot (SCGS) disease in India. Sugarcane (Saccharum officinarum L.) leaf samples with 'grassy shoot' symptoms were collected from the western region of Maharashtra state. The analysis based on restriction fragment length polymorphism (RFLP) profiles and 16S rRNA gene sequences indicated that most of the phytoplasma strains belong to the 16SrXI-B group. However, SCGS phytoplasma strain SC-Phy385 showed unique RFLP profiles with a similarity coefficient of 0.87 using online interactive phytoplasma classification tool, iPhyClassifier. The actual and virtual RFLP profiles of SC-Phy385 using amplified products of the 16S rRNA partial gene and its multiple sequence alignment with other RYD group phytoplasma sequences revealed that this strain was different from phytoplasma groups described earlier, representing new subgroup 16SrXI-F. The 16S rRNA gene-based phylogenetic analysis of reported RYD and BGWL group phytoplasmas showed that they are a genetically diverse group of phytoplasmas.

译文

在印度发现了一种新的植原体亚组菌株SC-Phy385 (GenBank登录号HF586648) 与甘蔗草芽 (SCGS) 病有关。从马哈拉施特拉邦西部地区收集了具有 “草芽” 症状的甘蔗 (Saccharum officinarum L.) 叶子样本。基于限制性片段长度多态性 (RFLP) 图谱和16S rRNA基因序列的分析表明,大多数植原体菌株属于16SrXI-B组。然而,SCGS植原体菌株SC-Phy385使用在线交互式植原体分类工具iphy分类器显示出独特的RFLP谱,相似系数为0.87。使用16S rRNA部分基因的扩增产物及其与其他RYD组植原体序列的多序列比对的SC-Phy385的实际和虚拟RFLP谱显示,该菌株与先前描述的植原体组不同,代表新的16SrXI-F亚群。对已报道的RYD和BGWL组植物质进行的基于16S rRNA基因的系统发育分析表明,它们是植物质的遗传多样性组。

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