The genetic network resulting in the production of an inflorescence is complex, involving one or more pathways including the photoperiod, maturity, gibberellin and autonomous pathways, and induction and repression of genes along the pathways. Understanding the cyclic expression profile of genes involved with photoperiod perception and floral pathway induction in sugarcane, an intermediate-short day plant (ISD), is crucial for identifying key genes and understanding how the profile changes in response to floral induction signals under decreasing daylengths. Homologues of 21 genes, and some gene alleles, associated with photoperiod perception and the flower induction pathway were examined in sugarcane variety Q174 over a 24-h light-dark cycle. The strongest expression of these genes was seen in the immature spindle leaves and levels of expression generally decreased with increasing leaf age. Significant changes in gene expression levels during a 24-h cycle were observed for 16 of the 21 genes tested. We have now defined an important baseline for expression patterns over a 24-h cycle in non-inductive conditions in sugarcane. These results can be utilised to select the optimal time for detecting changes during floral induction, differences between varieties that are responsive/non-responsive to photoperiod induction, and to identify genes that may be manipulated to enhance or inhibit flowering.

译文

导致花序产生的遗传网络是复杂的,涉及一个或多个途径,包括光周期,成熟度,赤霉素和自主途径,以及沿该途径诱导和抑制基因。了解甘蔗 (一种中短日植物 (ISD)) 中与光周期感知和花途径诱导有关的基因的循环表达谱,对于识别关键基因并了解在减少的情况下花诱导信号如何变化至关重要日长。在24小时的明暗周期中,在甘蔗品种Q174中检查了与光周期感知和花诱导途径相关的21个基因和一些基因等位基因的同源物。在未成熟的纺锤体叶片中观察到这些基因的最强表达,并且表达水平通常随着叶龄的增加而降低。在测试的21个基因中,有16个在24小时周期内观察到基因表达水平的显着变化。现在,我们已经为甘蔗在非诱导条件下24小时周期内的表达模式定义了重要的基线。这些结果可用于选择最佳时间,以检测花诱导过程中的变化,对光周期诱导有反应/无反应的品种之间的差异,并鉴定可以操纵以增强或抑制开花的基因。

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