The modification induced by interferon (IFN) in brown adipose tissue (BAT) was studied by high spatial resolution magnetic resonance imaging (MRI), histology, and transmission electron microscopy (TEM). In IFN-treated mice, at MRI, the interscapular BAT was slightly enlarged and showed non-homogeneous areas of lipid accumulation. The thickness of the subcutaneous white adipose tissue was reduced with respect to control mice. In the liver, MRI showed a lipid accumulation. In IFN-treated mice, by light microscopy, brown adipocytes showed a larger lipid deposit with respect to control mice. At TEM, in BAT, the mitochondria were reduced in number, smaller and the number of cristae was also significantly reduced with respect to the controls (9.1 +/- 1.5 vs 20.1 +/- 1.9, P < 0.01). The inclusions in the mitochondrial matrix were significantly less numerous in IFN-treated than in control animals (1.9 +/- 0.7 vs 0.9 +/- 0.7 for mitochondrial section, P < 0.01). Abnormalities of endoplasmic reticulum described in hepatocytes were not found in brown adipocytes of IFN-treated mice. The present work demonstrates that, in the BAT of suckling mice, IFN-treatment induces morphologic alterations and that brown adipocytes have MRI and TEM features resembling those found in the lipid laden BAT of aged animals.

译文

通过高空间分辨率磁共振成像 (MRI),组织学和透射电子显微镜 (TEM) 研究了干扰素 (IFN) 在棕色脂肪组织 (BAT) 中诱导的修饰。在IFN处理的小鼠中,在MRI上,肩胛间BAT略微增大,并显示出脂质积聚的非均质区域。相对于对照小鼠,皮下白色脂肪组织的厚度减少了。在肝脏中,MRI显示脂质积聚。在接受IFN处理的小鼠中,通过光学显微镜检查,相对于对照小鼠,棕色脂肪细胞显示出更大的脂质沉积。在TEM,在BAT中,相对于对照,线粒体数量减少,较小,并且cristae的数量也显着减少 (9.1/- 1.5 vs 20.1/- 1.9,P <0.01)。在IFN处理过的线粒体基质中的内含物明显少于对照动物 (线粒体切片的1.9/- 0.7 vs 0.9/- 0.7,P <0.01)。在IFN处理的小鼠的棕色脂肪细胞中未发现肝细胞中描述的内质网异常。目前的工作表明,在哺乳小鼠的蝙蝠中,IFN处理会诱导形态学改变,并且棕色脂肪细胞的MRI和TEM特征类似于老年动物的脂质蝙蝠中的MRI和TEM特征。

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